摘要
为了解吉林省羊附红细胞体病的流行情况,试验以羊附红细胞体26.0 ku抗原蛋白为诊断抗原,采用ELISA方法对吉林省的乾安、延吉、珲春、龙井4个县市的296份放牧绵羊的血清样本进行了羊附红细胞体抗体的检测。结果表明:在检测的296份血清样本中,以平原为主的乾安县羊附红细胞体抗体的阳性率为40.2%(53/132),以山区为主的延吉、珲春、龙井市羊附红细胞体抗体的阳性率分别为60.9%(39/64)、62.5%(25/40)、61.7%(37/60),平原与山区羊附红细胞体抗体阳性率差异极显著(P<0.01);羔羊与成年羊附红细胞体抗体阳性率差异极显著(P<0.01)。说明吉林省是羊附红细胞体病的流行地区,尤其在山区感染严重。
To understand the epidemic condition of eperythrozoonosis in sheep from Jilin Province, 296 serum samples which were randomly taken from sheep in the Qianan, Yanji, Hunchun and Longjing areas, were examined for antibodies to Eperythrozoon ovis （E. ovis） by ELISA, taking 26.0 ku antigen protein as a diagnostic antigen. The results showed that of the 296 samples, 40.2% （53/132） were positive for antibod- ies to E. ovis in Qianan which belonged to plain zone, 60.9% （39/64） ,62.5% （25/40） and 61.7% （37/60） were positive for antibodies to E. ovis in Yanji, Hunchun and Longjing which were mainly in mountain areas, respectively. The difference between plain and mountain areas was significantly highest（ P 〈0.01 ）in the positive rates of the antibodies to E. ovis, the difference between lamb and adult sheep was signifi- cantly highest（ P 〈 0.01 ） in the positive rates of the antibodies to E. ovis. It is concluded that Jilin Province is an endemic area for Eperythrozoonosis in sheep, especially in mountain areas with severe infection.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2012年第10期96-98,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
公益性行业(农业)科研专项(200903036-13)
延边大学大学生研究基金项目
关键词
羊附红细胞体
ELISA
流行病学调查
eperythrozoonosis in sheep
ELISA
epidemiological investigation
