摘要
本研究以a—^(32)P—dCTP标记的HPV—16基因组中三组亚基因片段为探针,应用制(?)内切酶技术和Southern印迹杂交方法进一步分析了9例HPV—16基因组已发生整合宫颈癌组织DNA中HPV—16基因组各亚基因片段存在的情况。结果表明,在整合状态下,PV—1基因组的部分L1 L2区和大部分E2 E4区是缺失的,而HPV—16基因组的E6 E7区■完整地被保留下来。提示HPV—16基因组的缺失区域很可能是其基因组发生断裂整合部位,而HPV—16基因组的E6 E7区可能是其导致细胞恶性转化的致癌基因。
It was further analyzed that the state of subgenomic fragments of HPV—16 genome in 9 cases of cervical cancer in which HPV—16 genome had been integrated into the genome of host cell by using labelled subgenomic fragments probes of HPR—16 genome and Sorthern blot hybribiazation method. The results showed that after HPV—16 genome integration ,the parts of L1 L2 regions and the majority of E2 E4 regions of HPV—16 genome were not deleted in some cervical cancer tissues,but the E6 E7 regions of HPV—16 genome were consistently intact in all cervical cancer tissues. These results suggest that the defective regions of HPV—16 genome may be its genomic integrating position, the E6 E7 regions of HPV—16 genome may be its oncogene causing malignant transformation of normal cervical epithelial cells.
出处
《合肥医学院学报》
1990年第1期6-9,共4页
Journal of Zunyi Medical University
关键词
宫颈癌
人乳头瘤病毒
亚基因片段
cervical cancer
human papilloma virus
subgenomic fragment
DNA probe
Southern blot hybridization
