摘要
目的:探讨血清中NKX2-1(NKX同源框-1)蛋白在原发性肺癌诊断中的临床价值。方法:2009年5月至2010年12月,检测以肺部肿块初次就诊的61例原发性肺癌患者(肺癌组)血清NKX2-1和癌胚抗原(CEA)蛋白的表达,并与49例健康成年人(正常对照组)比较,验证NKX2-1蛋白诊断肺癌的准确性,分析NKX2-1在原发性肺癌中的诊断价值。结果:肺癌组血清NKX2-1蛋白表达(1.4206±0.1257)ng/ml高于正常对照组(0.7646±0.0734)ng/ml,两者比较差异有统计学意义(P<0.01);经ROC分析显示:NKX2-1对原发性肺癌有较好的诊断准确性(曲线下面积为0.859);Kappa验证NKX2-1蛋白(Kappa值为0.586)对原发性肺癌的诊断一致性高于CEA(Kappa值为0.396),而且NKX2-1结合CEA蛋白可提高肺癌诊断的准确性(Kappa值为0.704;灵敏度及特异度分别为83.6%和87.0%)。结论:血清NKX2-1蛋白是诊断原发性肺癌较为敏感和特异的指标;NKX2-1与CEA结合可提高原发性肺癌诊断的准确性。
Objective： To evaluate serum Nkx2-1（NKX homeobox-1） levels in diagnosis of primary lung cancer.Methods： The serum NKX2-1 and CEA（carcinoma embryonic antigen） levels were measured in 61 patients with primary lung cancer admitted from May 2009 to December 2010 and 49 healthy individuals served as controls.The receiver operating characteristic curve（ROC） of NKX2-1 in diagnosis for primary lung cancer was analyzed.The value of serum NKX2-1 in diagnosing primary lung cancer was compared with that of CEA by χ2 test and Kappa test.Results： The serum Nkx2-1 levels in lung cancer were significantly higher than those in controls [（1.4206±0.1257）ng/ml vs（0.7646±0.0734）ng/ml,P0.01].ROC analysis showed the area under the curve of serum NKX2-1 was 0.859.The Kappa value of NKX2-1 was higher than that of CEA（0.586 vs 0.396,P0.05）.Combination of serum NKX2-1 with CEA improved the Kappa value to 0.704,and also had high sensitivity（83.6%） and specificity（87.0%） for diagnosis of primary lung cancer.Conclusions： Serum NKX2-1 protein can be used as a marker for diagnosis of lung cancer,the combination of NKX2-1 with CEA may further improve the diagnostic value.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2012年第5期535-539,共5页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金(30670929)
浙江省医药卫生科技计划项目(2010KYB061)
