摘要
利用柱状黄杆菌为刺激物,对褶纹冠蚌进行注射感染,在注射后3、6、12、24、48h后分别取血清、肝胰腺,测定其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)的活性和谷胱甘肽(GSH)、过氧化氢(H2O2)、丙二醛(MDA)的含量的变化。结果表明,经柱状黄杆菌感染后,实验组血清中的SOD活性在6h时与对照组有显著性差异;GPX的活力在3h与对照组有明显的差异,GSH的含量在24h明显低于对照组。而实验组肝胰腺中GPX的活力在24和48h时显著高于对照组。实验组血清和肝胰腺中MDA和H2O2的含量与对照组没有明显的差异。褶纹冠蚌注射柱状黄杆菌感染后,血清中各抗氧化指标比肝胰腺中的变化大。
This paper investigated the activities of antioxidant enzymes in haemolymph and hepatopancreas from Cristaria plicata infected by Flavobacterium columnare.Blood serum and hepatopancreas were extracted at 3,6,12,24 h and 48 h from the C.plicata injected with F.columnare（109 cell/mL） and 0.9% NaCl solution（control group）,respectively.The levels of Superoxide dismutase（SOD）,Glutathione peroxidase（GPX）and the content of glutathione（GSH）,hydrogen peroxide（H2O2） and malonaldehyde（MDA） were tested.After C.plicata was injected by F.columnare,the activities of SOD from blood serum were significantly different from control at 6 h,and the activities of GPX from blood serum were significantly different from control at 3 h,too.Meanwhile,the activities of GPX from hepatopancreas were higher than those in control at both 24 and 48 h.The content of GSH from blood serum was dramatically lower than that in control at 24 h.The content of MDA and H2O2 from blood serum and hepatopancreas had obvious changes.The indexes from blood serum changed more than those from hepatopancreas.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2012年第4期389-393,共5页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(30960296)
江西省教育厅科技资助项目(GJJ12024)
江西省科技攻关资助项目(2004)
关键词
褶纹冠蚌
柱状黄杆菌
酶活性
Cristaria plicata
Flavobacterium columnare
enzymatic activities
