摘要
目的:建立三七药材、破壁粉体和破壁饮片的HPLC指纹图谱,为三七破壁饮片的质量控制提供依据。方法:采用高效液相色谱法,Hypersil ODS2色谱柱,流动相:乙腈-水(梯度洗脱),流速:1.0 mL/min,检测波长:203nm,柱温:25℃。采用"中药色谱指纹图谱相似度评价系统"软件对图谱进行分析处理。结果:三七药材、破壁粉体和破壁饮片皂苷类成分指纹图谱标定8个共有峰,三七药材、破壁粉体和破壁饮片之间相似度均大于0.9。结论:建立的HPLC指纹图谱方法具有良好的精密度、重复性和稳定性,适用于三七破壁饮片的质量控制。
Objective：To establish the HPLC fingerprint chromatograms of crude Notoginseng,cell wall-broken powder and cell wall-broken decoction pieces of Notoginseng and provide evidence for quality control of cell wall-broken decoction pieces of Notoginseng.Methods：The HPLC procedure was performed on the chromatographic column of Hypersil ODS2,and the mobile phase was acetonitrile and water in gradient elution with the flow velocity of 1.0 mL/min.The detection wavelength was 203 nm and the column temperature was 25 ℃.The chromatograms was analyzed with the software of ＂similarity evaluation system for chromatographic fingerprint of TCM＂.Results：Eight common peaks were pinpointed from the chromatograms of different batches of crude Notoginseng,cell-broken powder and cell-broken decoction pieces,the similarities of the chromatograms were all larger than 0.9.Conclusion：The method of the HPLC fingerprint chromatogram is of good precision,reproducibility and stability,which is suitable for quality control of cell wall-broken decoction pieces of Notoginseng.
出处
《中药材》
CAS
CSCD
北大核心
2012年第7期1056-1061,共6页
Journal of Chinese Medicinal Materials
基金
广东省粤港关键领域重点突破招标项目(20080106-2)
广东省科技计划项目(粤科计字[2008]72号)
中山市科技强企支撑计划项目(20092A113)
关键词
三七药材
破壁粉体
破壁饮片
指纹图谱
皂苷
Crude Notoginseng
Cell wall-broken powder
Cell wall-broken decoction pieces
Fingerprint chromatogram
Saponins
