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转基因水稻中过量表达OsHPT提高胚乳中生育酚含量 被引量：4

Increase of Tocopherol Content in Endosperm by Overexpression of Homogentisate Phytyltransferase in Transgenic Rice
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摘要 为了提高稻米中生育酚的含量,本研究经农杆菌介导将由CaMV35S启动子驱动的水稻尿黑酸植基转移酶基因(OsHPT)导入粳稻品种武育粳3号,经RT-PCR等分析证明导入基因能在水稻胚乳中表达。HPLC测定结果表明,水稻中过表达OsHPT后,转基因稻米胚乳中总生育酚的含量极显著高于未转化对照,且主要表现为α-生育酚含量的提高,总生育酚和总维生素E含量最高可分别提高1.5和0.8倍。 In order to increase the tocopherol content of rice, the homogentisate phytyltransferase （OsHPT） gene was transferred into an elite rice japonica cultivar Wuyujing3 driven with CaMV 35S promoter by Agrobacterium-mediated transformation RT-PCR analyses confirmed the expression of the input transgene in rice endosperm. HPLC results showed that after OsHPT was expressed in the rice, the total tocopherol content in endosperm of transgenic rice lines was significantly higher than the wild type, mainly for the increase in α-tocopherol Content, In transgenic rice, the contents of total tocopherol and vitamin E increased maximally 2.5 and 1.8 folds respectively compared with those of wild type.
作者 张桂云 刘如如 张鹏 徐根 朱姜 梁国华 刘巧泉
机构地区 扬州大学农学院 国家洗漱用品质量监督检验中心
出处 《分子植物育种》 CAS CSCD 北大核心 2012年第5期528-534,共7页 Molecular Plant Breeding
基金 转基因生物新品种培育重大专项(2011ZX08001-006)资助
关键词 转基因水稻 维生素E 生育酚 尿黑酸植基转移酶 Transgenic rice, Vitamin E, Tocopherol, Homogentisate phytyltransferase
分类号 S511 [农业科学—作物学]
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参考文献37

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二级参考文献24

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分子植物育种
2012年 第5期

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