摘要
目的考察微粉化对制马钱子中士的宁、马钱子碱溶出度的影响。方法采用《中华人民共和国药典》2010年版二部附录溶出度浆法,用HPLC测定,分别在人工胃液和人工肠液(无酶)模拟体内环境的溶出介质下,转速50r/min,体积为900 mL,比较微粉化前后马钱子粉的溶出行为。结果以T50、Td、T90为溶出度参数,在人工肠液中,以马钱子碱、士的宁为考察指标,马钱子超微粉分别为0.38、1.33、19.50,0.39、1.31、17.63 min;马钱子粗粉分别为19.43、44.53、249.12,20.84、44.96、229.95 min。在人工胃液中,以马钱子碱、士的宁为考察指标,马钱子超微粉分别为0.32、1.25、20.60,0.45、1.41、20.81 min;马钱子粗粉分别为20.53、43.31、220.30,20.76、46.02、241.82 min,两者比较差异均有统计学意义(P<0.01)。结论微粉化能提高马钱子中士的宁、马钱子碱的溶出量与溶出速度。
Objective To investigate the effects of micronization on the dissolution of strych- nine and brucine in semen strychni. Methods According to the dissolution method described in the appendix XC of Chinese Pharmacopoeia （2010 edition） , artificial gastric fluid and intestinal fluid as dissolution medium with the rotating speed at 50 r/min, and the sampling volume was 900 mL,dissolution of micronization of semen strychni before and after was determined by HPLC. Results T50, Td and %o were selected as dissolution parameters, brucine and strychnine as indica- tors, the dissolution parameters of uhrafine powder of semen strychni were 0.38, 1.33, 19.50,0.39, 1.31, 17.63 min;coarse powder were 19.43,44.53,249.12,20.84,44.96,229.95 min in artificial in- testinal fluid. And in artificial gastric fluid, the dissolution parameters of uhrafine powder of strychni semen were 0.32, 1.25, 20.60,0.45, 1.41, 20.81 rain;coarse powder were 20.53, 43.31, 220.30,20.76, 46.02, 241.82 min. The dissolution parameters of strychnine and brucine of uhrafine powder and its coarse powder in gastric and intestinal fluid had a statistically significant difference （P〈0.05）. Conclusion Micronization can improve dissolution content and dissolution rate of strych- nine and brucine in strychni semen.
出处
《湖南中医药大学学报》
CAS
2012年第7期37-40,共4页
Journal of Hunan University of Chinese Medicine
基金
湖南省自然科学基金重点项目(09JJ3055)
湖南省科技厅资助项目(20107D2004
2009XK-6016)
关键词
微粉化
马钱子
士的宁
马钱子碱
溶出度
micronization
semen strychni
strychnine
brucine
dissolution
