摘要
[目的]探讨SOD活性与玉米对玉米大斑病的抗性的关系。[方法]采用菌盘接种法将不同致病性的玉米大斑病菌YC和01-23T接种玉米叶片,并测定被侵染后玉米的SOD活性的动态变化。[结果]接种玉米大斑病菌强毒菌株YC和弱毒菌株01-23T后,在1~5 d内玉米叶片SOD活性表现出明显的阶段性:1~3 d逐渐降低,3~4 d逐渐回升,4~5 d又下降;但在1~4 d内,接种处理的玉米叶片SOD活性均高于未接种处理,第5天才显著降低。病菌致病性不同,引起的玉米叶片SOD活性变化不同,其中弱毒菌株01-23T侵染第1天SOD活性极显著高于强毒菌株YC侵染,但2~3 d时与强毒菌株YC无显著差异。[结论]SOD活性对于玉米的抗病菌初始侵染能力具有非常重要的作用,但在发病后抗病菌扩展方面的作用不显著。
[Objective] The paper was to explore the relationship between SOD activity and resistance of corn against Setosphaeria turcica.[Method] Strain YC and 01-23T of S.turcica with different pathogenicity were inoculated into corn leaves by fungal disc inoculation method,and the dynamic changes of SOD activity in infected corn leaves were measured.[Result] After inoculation of strong virulent strain YC and weak virulent strain 01-23T of S.turcica,SOD activity in corn leaves within 1-5 d showed obvious stages,which gradually decreased within 1-3 d,gradually increased within 3-4 d,and decreased again within 4-5 d.The SOD activity of corn leaves in inoculation treatment was higher than that in the treatment without inoculation within 1-4 d,which significantly decreased at the fifth day.The pathogen with different pathogenicity would lead to different changes in SOD activity of corn leaves.SOD activity of corn leaves infected by weak virulent strain 01-23T for the first day was extremely higher than that infected by strong virulent strain YC,but had no significant difference with strong virulent strain YC at 2-3 d.[Conclusion] SOD activity played an important role in initial resistant ability of corn against pathogen infection,and had no significant effect on resistance against pathogen extension after incidence.
出处
《安徽农业科学》
CAS
2012年第28期13821-13823,共3页
Journal of Anhui Agricultural Sciences
基金
河北省自然科学基金项目(C2010001854)
唐山市科技局资助项目(10120201A-7)
关键词
玉米
玉米大斑病
SOD活性
抗病性
Corn
Setosphaeria turcica
SOD activity
Pathogenicity