摘要
[目的]探讨培养基营养条件对安徽省大豆根腐病病原菌菌丝生长和孢子形成的影响。[方法]研究了不同培养基、碳源、氮源对安徽地区大豆根腐病主要致病菌——茄病镰孢(Fusarium solani)生长速率及产孢量的影响。[结果]在供试的7种培养基中,该菌在PDA培养基上生长最好,产孢量在查彼培养基上最高;该菌在以蔗糖为碳源的培养基上生长最快,产孢量最高;在以尿素为氮源的培养基上生长最快,产孢量在以硝酸铵为氮源的培养基上最高。[结论]为该菌的相关研究以及所致大豆根腐病的综合防治提供了理论依据。
[ Objective ] The aim was to explore effects of medium nutrients on mycelial growth and eonidial production of soybean root rot pathogens in Anhui province. [ Method ] Effects of different solid mediums, carbon sources and nitrogen sources on the mycelial growth and eonidial production of Fusarium solani which was the main pathogen of soybean root rot in Anhui province were investigated. [ Result ] The better media for myeelial growth of F. solani was PDA media among the 7 tested media. The optimum medium for conidial production was Czapek medium. For myeelial growth and conidial production on solid media, the optimum carbon source was sucrose. The optimum nitrogen sources for mycelial growth was urea, and the optimum nitrogen sources for conidial production was NH4NO3. [ Conclusion] The research pro- vides theoretical basis for relative study on F. solani and comprehensive treatnent of caused soybean root rot.
出处
《安徽农业科学》
CAS
2012年第28期13807-13808,13810,共3页
Journal of Anhui Agricultural Sciences
基金
国家公益性行业(农业)科研专项(3-20)
国家大豆产业技术体系项目(Nycytx-004)
关键词
茄病镰孢
培养基
碳源
氮源
生长速率
产孢量
Fusarium solani
Medium
Carbon source
Nitrogen source
Growth rate
Conidial production
