摘要
84只1日龄小公鸡,分为四组,分别为对照组、新城疫弱毒疫苗免疫组(ND组)、饲喂半胱胺的试验组(CS组)以及饲喂半胱胺后,再用新城疫弱毒疫苗进行消化道粘膜的试验组(CN组).应用甲苯胺蓝染色技术研究了十二指肠中肥大细胞数量的变化.结果显示:新城疫疫苗首免后第三周,CN组十二指肠中肥大细胞数量比ND组明显增加(P<0.01);首免后第五周,各试验组十二指肠中肥大细胞的数量均比对照组多,尤其是CN组,可能因为个体差异较大,各组间并无显著差异.本试验的结果提示,肥大细胞的数量可能反映了消化道粘膜的免疫水平.
Eight-four 1-day-old cock were divided into control group, ND treated group, CS treated group and the group was immunized with Newcastle disease attenuated vaccine by digestive rout after feeding cysteamine（CN group）. Toluidine blue staining method was used to study the change of mast cells number in chicken small intestinal. The results showed that the number of mast cells in duodenum of CN group increased significantly （P〈0.01） compared to the group immunizing only with Newcastle disease attenuated vaccine at 3 week after oral immunization; Comparing to the control group at 5 week, the number of mast cells among experiment group increased significantly, though no differences existed among all groups. Our results suggest the number of mast cells might reflect the digestive mucosal immunization.
出处
《湖南文理学院学报（自然科学版）》
CAS
2012年第3期33-35,45,共4页
Journal of Hunan University of Arts and Science(Science and Technology)
基金
国家自然科学基金项目(30070564)
关键词
半胱胺
鸡
十二指
肥大细胞
cysteamine
chicken
duodenum
mast ceils
