摘要
目的对67例胃肠道间质瘤(GISTs)患者(病例组)及39例非GISTs患者(对照组)的肿瘤标本进行免疫组化检测,比较GISTs患者DOG1蛋白和CD117蛋白的表达情况,探讨DOG1蛋白在GISTs诊断中的价值。方法应用免疫组化方法检测DOG1蛋白和CD117蛋白在病例组及DOG1蛋白在对照组的表达情况,分析DOG1蛋白和CD117蛋白表达与GISTs患者临床病理特征的关系。结果对照组中3例患者DOG1蛋白呈弱阳性表达,阳性表达率为7.69%(3/39);病例组中64例患者DOG1蛋白呈阳性表达,阳性表达率为95.52%(64/67),两组DOG1蛋白阳性表达率比较,差异有统计学意义(χ2=82.0029,P<0.01)。GISTs患者DOG1蛋白阳性表达率(95.52%)高于CD117蛋白阳性表达率(92.54%,62/67),但差异无统计学意义(χ2=0.5317,P>0.05);在CD117蛋白表达阴性的5例患者中,DOG1蛋白均呈阳性表达。不同性别、年龄、肿瘤部位、肿瘤直径、组织学类型及危险度的GISTs患者DOG1蛋白及CD117蛋白阳性表达率比较,差异均无统计学意义(P>0.05)。结论 DOG1蛋白是一种新的较敏感和特异的GISTs标记物,在胃肠道间叶源性肿瘤的诊断及鉴别诊断中具有一定应用价值,与CD117蛋白联合应用可起到有效的互补作用,可提高GISTs的诊断率,且检测方法简便易行,值得临床推广应用。
Objective To investigate the expression of DOG1 protein in patients with gastrointestinal Stromal Tumors （GISTs） and explore its role in the diagnosis. Methods Totally 67 GISTs patients （ GISTs group） and 39 non - GISTs patients （control group） were enrolled in this study. The expressions of DOG1 and CDII7 were detected using immunohistochcmical methods and compared in both groups. Results The expression rates of DOG1 and CD117 were 95.52% （64/67） and 92. 54% （ 62/67 ） in the GISTs group and showed no correlation with age, gender, tumor location, tumor size, histological type and risk grade （P 〉0. 05）. The expression rate of DOG1 was 7.69% （3/39） in the control group, which was significantly lower than that in the GISTs group （ P 〈 0.01 ）. Conclusion DOG1 protein is a novel sensitive and specific GISTs marker and can be helpful in the diagnosis and differential diagnosis of GISTs, especially when combine with CD117.
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第26期3025-3027,共3页
Chinese General Practice
