摘要
以3个栽培海岛棉品种新海30、新海24、新海16诱导而来的胚性愈伤组织为受体材料,分析了3种农杆菌菌株EHA105、LBA4404、GV3101和3种已构建质粒pCAMBIA1301、pCAMBIA1304、pBI121对已建立的农杆菌介导海岛棉外源基因转化体系的影响。研究结果表明,菌种和质粒对转化率有极其显著的影响,而两者之间的互作关系也比较显著。最终得到农杆菌介导转化海岛棉胚性愈伤组织的最佳菌种和质粒是GV3101和pBI121。
This study was conducted to analyze the influence of 3 agrobacterium-mediated plants and 3 plasmids：pCAMBIA1301 ,pCAMBIA1304, pBI121 on the established exogenous gene transformation sys- tem of agrobacterium-mediated sea island cotton, by using the embryogenesis callus of Xinhai 16, Xinhai 24 and Xinhai 30 as receptor materials. The resutls indicated that agrobacterium-mediated and plasmid had significant influence on gentic transformation rate and there were significant mutual relations between the two. Eventually,the best agrobacterium-mediated and plasmid of mbryogenic callus of the sea island cotton were obtained：GV3101 and pBI121.
出处
《新疆农业大学学报》
CAS
2012年第4期302-305,共4页
Journal of Xinjiang Agricultural University
基金
国家自然科学基金项目(30960201
30700513)
关键词
海岛棉
菌种
质粒
遗传转化
胚性愈伤组织
sea island cotton
agrobacteriu-mediated
plasmid
genetic transformation
embryonic callus
