摘要
目的探讨乳腺上皮粘蛋白(SBEM)和人乳腺珠蛋白(hMAM)在乳腺癌中的表达及其临床意义。方法采用实时荧光定量PCR(RT-PCR)检测109例乳腺癌患者和25例对照组(5例健康者与20例其他肿瘤患者)外周血SBEM mR-NA和hMAM mRNA的表达情况,流式细胞术检测SBEM和hMAM阳性表达细胞的比例;并分析其与临床病理特征的关系。结果 RT-PCR检测显示SBEM mRNA和hMAM mRNA仅表达于乳腺癌患者外周血中,在健康者和其他肿瘤患者外周血中无表达。流式细胞术检测显示SBEM和hMAM在乳腺癌患者中阳性表达细胞的比例分别为55.9%和40.4%,而在健康者和其他肿瘤患者中仅为10%左右。乳腺癌外周血SBEM和hMAM表达与TNM分期、淋巴结转移有关,而与年龄、肿瘤大小、激素受体和C-erbB-2均无关。结论 SBEM mRNA和hMAM mRNA在乳腺癌患者外周血中均有高表达,两者联合检测有助于判断血道微转移的存在。
Objective To explore the expression and clinical significance of small breast epithelial mucin （SBEM） and hu- man mammaglobin（hMAM） in breast cancer patients. Methods The expression of SBEM mRNA and hMAM mRNA in peripheral blood samples of 109 primary breast cancer patients and 25 controls （ 5 healthy donors and 20 other tumor patients ） were detected by RT-PCR. The percentages of SBEM ＋/hMAM ＋ cell were measured by flow cytometry （ FCM ）. The relationship between them and clinicopathologie features were analyzed. Results RT-PCR results showed that SBEM mRNA and hMAM mRNA only expressed in pe- ripheral blood of breast cancer patients, while no expression was found in that of controls. FCM showed that the percentages of SBEM ＋ and hMAM ＋ cells in peripheral blood were 55.9% （61/109） and 40. 4% （44/109） in breast cancer, much higher than 10% in controls. Both SBEM mRNA and hMAM mRNA expression were related to TNM stage and lymph node metastasis, but not to age, tumor size, hormone receptor and C-erbB-2. Conclusion SBEM mRNA and hMAM mRNA highly expressed in peripheral blood of breast cancer, and the united detection of them may be helpful to judge hematogenous micrometastasis of breast cancer.
出处
《临床肿瘤学杂志》
CAS
2012年第9期809-813,共5页
Chinese Clinical Oncology
