摘要
目的检测乳腺癌组织中褪黑素受体(MT1)与CUE结构域蛋白2(CUEDC2)的表达,探讨二者表达的相关性及临床意义。方法采用Western blotting和免疫组化SP法检测80例乳腺浸润性导管癌、30例乳腺导管内癌和30例正常乳腺组织中MT1和CUEDC2的表达情况。结果 MT1在乳腺浸润性导管癌、乳腺导管内癌、正常乳腺组织中的阳性表达率分别为78.8%、60.0%和16.7%,三者差异有统计学意义(P<0.05);CUEDC2在乳腺浸润性导管癌、乳腺导管内癌的阳性表达率分别为68.8%、53.3%,明显高于正常乳腺组织的13.3%,差异有统计学意义(P<0.05)。Western bloting半定量检测显示,乳腺浸润性导管癌MT1和CUEDC2表达分别为0.542±0.078和0.461±0.057,均高于正常乳腺组织(P<0.05)。MT1和CUEDC2表达与乳腺浸润性导管癌患者有无淋巴结转移(P=0.034,P=0.027)和组织学分级(P=0.018,P=0.022)均有关,但与年龄和肿瘤大小无关(P>0.05)。乳腺浸润性导管癌中MT1表达与CUEDC2表达呈正相关(r=0.441,P<0.05)。结论MT1和CUEDC2高表达与乳腺癌的发生、发展及转移有关,联合检测二者表达可为乳腺癌基因治疗寻找新的干预靶点提供实验依据。
Objective To explore the relationship and clinical significance between the expressions of melatonin receptor （MT1） and CUE domain-containing 2 （CUEDC2） in breast cancer. Methods Western blotting and immunohistochemieal method were performed to detect the expression of MT1 and CUEDC2 in 80 invasive duetal breast cancer, 30 intraductal breast cancer and 30 normal breast tissues. Results The positive expression rates of MT1 in invasive duetal breast cancer, intraductal breast cancer and normal breast tissue were 78.8% ,60. 0% and 16. 7% with statistical significance （P 〈 0. 05 ）. The positive expression rate of CUEDC2 in invasive duetal breast cancer and intraductal breast cancer tissue was 68.8% and 53.3% , higher than 13.3% in normal breast tis- sue（P 〈 0. 05）. Western blotting showed expressions of MT1 and CUEDC2 in invasive duetal breast cancer were 0. 542±0. 078 and 0. 461 ± 0. 057, significantly higher than those in normal breast tissue（P 〈 0. 05 ）. The positive expressions of MT1 and CUEDC2 in invasive ductal breast carcinoma was correlated with axillary lymphnode metastasis （P = 0. 034, P = 0. 027） and histological grade （P = 0. 018, P = 0. 022 ） , but not with age and tumor size of patients （ P 〉 0. 05 ）. Positive correlation was observed between the expression of MTI and CUEDC2 in invasive ductal breast cancer（ r = 0. 44,P 〈 0. 05）. Conclusion The overexpression of MT1 and CUEDC2 is re- lated with the occurrence,development and metastasis of breast carcinoma. The combined detection of their expression provides experi- mental evidence for finding the new intervention targets in the breast cancer gene therapy.
出处
《临床肿瘤学杂志》
CAS
2012年第9期795-800,共6页
Chinese Clinical Oncology
基金
江苏省镇江市社会发展资助项目(SH2007048)
