摘要
以3个不同发育时期的‘糯米糍’荔枝果皮和幼嫩叶片为试材,对其ANS基因进行了克隆及分析。结果表明:花青素合成酶(Anthocyanidin synthase,ANS)是花色素苷生化合成途径中的一个关键酶。利用同源克隆方法从荔枝果皮中克隆得到了1个ANS基因,该基因开放阅读框的长度为1 074bp,编码357个氨基酸。以基因组为模板,扩增得到了1 279bp的核苷酸序列与cDNA序列比对发现,基因组序列中还有1个内含子,位置在504～708bp之间。通过系统发育分析发现,该基因编码的蛋白与葡萄、可可豆、柑橘等聚为一类。
Taking the peel and young leaves of Litechi chinensis ‘Nuomici’at three different stages as material,its ANS gene was cloned and analyzed.The results showed that the anthocyanidin synthase（ANS） was a key enzyme in the anthocyanin biosynthesis.AANSgene was cloned from litchi pericarp by homologous cloning method.The open reading frame was 1 074bp,and encoding 357amino acids.The 1 279bp length fragment was amplified from genome and analysis,which contained five introns,respectively,in 504～708bp.It was found that the gene encoding for the protein had close relationship with mountain grape（Vitis vinifera）,cocoa（Theobroma cacao）,citrus（Citrus sinensis）,and other fruit trees through phylogenetic analysis.
出处
《北方园艺》
CAS
北大核心
2012年第19期118-121,共4页
Northern Horticulture
基金
国家科技支撑计划资助项目(2006BAD01A1705)
现代农业产业技术体系建设专项资金资助项目(nycytx-32)
国家自然科学基金资助项目(30971985)
关键词
荔枝
ANS
基因克隆
序列分析
Litchi chinensis Sonn.
ANS
gene cloning
sequence analysis
