摘要
目的探讨单酰甘油脂肪酶(monoacylglycerol lipase,MAGL)在原发性肝细胞肝癌中的表达及临床意义。方法应用Real-Time PCR、免疫组织化学和Western blot检测50例肝癌及癌旁组织、16例正常肝组织标本中MAGL的表达,比较MAGL在癌组织、癌旁组织及正常肝组织中表达的差异,并分析其与临床病理特征的关系。结果 (1)免疫组织化学检测结果显示,MAGL在癌组织主要表达于细胞核中,而在癌旁组织和正常组织主要表达在细胞质中;MAGL在肝癌组织、癌旁组织及正常肝组织三组中差异有统计学意义(P<0.05);MAGL在低分化肝癌中的表达阳性率明显高于高分化肝组织(P<0.05)。(2)Real-Time PCR结果显示,高分化、低分化以及癌旁组织中MAGL的mRNA水平均较正常肝组织中显著增高(P<0.01);(3)Western blot结果显示,癌组织中MAGL蛋白呈强阳性表达,而癌旁组织和正常组织中呈弱阳性表达;高分化及低分化癌组织中MAGL的蛋白水平较癌旁组织和正常肝组织中显著增高,组间比较差异有统计学意义(P<0.01)。结论 MAGL表达水平与原发性肝细胞肝癌的恶性表型呈正比,它可能在癌细胞侵袭和转移过程中发挥重要作用。
Objective To evaluate the expression and clinical significance of monoacylglycerol lipase （MAGL） in primary hepatocellular carcinoma （HCC）. Methods The MAGL expression of cancer tissues and the adjacent non - tumor tissues from 50 HCC cases, and of hepatic tissues from 16 normal livers, were assessed with Real - Time PCR, im- munohistochemistry and Western Blot. The correlation between the MAGL expression and pathological characteristics were also analyzed. Results （ 1 ） According to immunohistochemetry, MAGL was mainly expressed in nucleus in cancer tis- sues, while cytoplasmic expression was dominated in the adjacent non - tumor tissues and normal hepatic tissues. In addi- tion, there were significant differences in MAGL expression among cancer tissues, adjacent non - tumor tissues and normal tissues （ P 〈 0. 05 ）, furthermore, the MAGL expression was significantly higher in poorly differentiated liver cancer tissue than that in well differentiated liver tissues （ P 〈 0.05 ）. （2） According to real - time PCR, the MAGL mRNA was signif- icantly evaluated in either poor differentiated or high differentiated cancer tissues, when comparing with that in normal he- patic tissues （P 〈 0.05 ）. （3） According to Western Blot, strong positive MAGL expression was observed in cancer tis- sues, while weak positive MAGL expression was revealed in adjacent non -tumor tissues and normal tissues. Further- more, the MAGL expression was significantly up - regulated in either poor or high differentiated hepatic cancer tissues when comparing with that in both adjacent non - tumor and normal hepatic tissues （ P 〈 0. 01 ）. Conclusion The up - regulation of MAGL is positively correlated with the malignancy of primary hepatocellular carcinoma, which probably plays an important role in invasions and metastasis of cancer cells.
出处
《广东医学》
CAS
CSCD
北大核心
2012年第18期2746-2749,共4页
Guangdong Medical Journal
基金
重庆市医学科研计划项目(重点项目)(编号:2011-1-123)
关键词
单酰甘油脂肪酶
肝细胞肝癌
脂代谢
monoacylglycerol lipase
hepatocellular carcinoma
lipid metabolism
