摘要
目的:研究化合物1,8-二羟基-3-乙酰基-6-甲基-9,10蒽醌抑制卵巢癌细胞SKOV3侵润转移作用。方法:利用细胞划痕实验、MTT法、明胶酶谱实验等方法,研究1,8-二羟基-3-乙酰基-6-甲基-9,10蒽醌对SKOV3细胞侵润、黏附和迁移能力的影响。结果:1,8-二羟基-3-乙酰基-6-甲基-9,10蒽醌具有抑制SKOV3细胞运动迁移能力和抑制SKOV3细胞的黏附能力;无细胞毒作用浓度的1,8-二羟基-3-乙酰基-6-甲基-9,10蒽醌处理SKOV3细胞后,基质金属蛋白酶MMP-2、MMP-9的活性均有一定程度的降低。结论:1,8-二羟基-3-乙酰基-6-甲基-9,10蒽醌具有抑制SKOV3细胞侵润、黏附和迁移能力。
Objective： To observe the inhibitive effects of 1,8-dihydroxy-3-acetyl-6-methyl-9, 10 anthracenone on invasive and metastasis of ovarian carcinnoma cells SKOV3. Methods： Gelatin zymography, scratch movement assay in vitro and MTT assay were employed to study the mechanisms of the effects of 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthracenone. Results： The migration movement ability, adhesion ability and MMP-2, MMP-9 levels in SKOV3 were all inhibited by the nontoxic concentration of l, 8-dihydroxy-3-acetyl-6-methyl-9,10 anthracenone. Conclusion： Compared with carboplatin, 1,8-dihydroxy-3- acetyl-6- methyl-9,10 anthracenone shows stronger inhibitive effects on invasive and metastasis activity of SKOV3 cells.
出处
《广西医科大学学报》
CAS
2012年第4期496-498,共3页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81060270
No.81060218)
