摘要
采用PCR-SSCP、PCR-RFLP技术对6个猪群共计276头猪的POU1F1基因的4个位点的多态性进行多态性分析,在针对Intron1设计的2对引物扩增的片段中分别发现1处突变位点,各存在2个等位基因(A和B、C和D);在引物P2位点,海南4个品系的猪种(五指山猪、五指山猪近交系、临高猪、屯昌猪)均没有发现多态性,而在滇南小耳猪、香猪2个猪种中BB基因型频率较AA基因型频率占优势,群体遗传多态性检测处于中度多态;在引物P3位点,在6个猪群体中,DD基因型频率较CD基因型频率占优势,群体遗传多态性检测均处于中度多态。SPSS软件分析五指山猪POUIF1基因P3位点的多态性与3个生长阶段及其日增重的关联程度,结果表明P3位点不同基因型对五指山猪3个阶段的生长及日增重间均无显著差异。
Six Chinese local pig breeds were used as experimental examples, a mutation in intron of pig Pit - 1 gene was fourld by using PCR-SSCP analysis. The result of population genetics analyses showed that in the mutation of P2, the frequency of BB genotype was significantly higher than that of AA genotype in Diannan Small ear pig and Xiang pig and there was no polymorphism in Hainan native pigs. In the mutation of P3, the frequency of CD genotype was significantly higher than that of DD genotype and had intermediate polymorphism in the six Chinese local breeds. The least square analysis showed that the different genotypes of intronl of POUIF1 gene did not affect WZSP body weight of 2 month,4 month,8 month and daily gain.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第4期775-780,808,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
海南省自然科学基金项目(309018)
海南省重点科技项目(琼财预[2005]1173号)
海南省科学事业费项目(琼财预[2010]139号)
