摘要
研究建立了单层培养,双层显色分离产生物胺肠细菌和乳酸菌的方法,并分离到96株产生物胺肠细菌和58株产生物胺乳酸菌。经变性凝胶梯度电泳分析(PCR-DGGE)和PCR扩增测序后与Genebank数据库比对得知,这154株产胺菌属于5种菌,分别为屎肠球菌、粪肠球菌、阴沟肠杆菌、大肠埃希杆菌和产气肠杆菌。并采用特异性引物证明了产生物胺基因的存在。
A new method of monolayer cultivation coupled with double-layer color development was established. 96 strains of biogenic amines-producing lactic acid bacteria and 58 strains of biogenic amines-producing Enterobacteria were obtained. The 154 strains of biogenic amines-producing bacteria belonged to 5 species according to the results of polymerase chain reaction denaturing gradient gel eleetrophoresis （PCR-DGGE） analysis. After sequencing of PCR- products of 16S rDNA fragments and alignment in Genebank, the 5 strains of biogenic amines-producing bacteria were found to be Enterococcus faecium, Enterococcus faecalis, Enterobacter cloacae, Escherichia coli, and Enterobacter aero- genes. The presence of Biogenic amines-producing gene was demonstrated by special primers.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第8期47-52,共6页
Food and Fermentation Industries
基金
新疆熏马肠生物胺产生和累积机理研究(项目批准号:31160329)
石河子大学自然科学重点项目:新疆熏马肠中生物胺累积机理研究(项目编号:ZRKX2010ZD04-3)
关键词
生物胺
肠细菌
乳酸菌
分离鉴定
biogenic amines, Enterobacteria, lactic acid bacteria, isolation and identification
