摘要
目的研究风轮菜活性部位(CCE)对大鼠离体胸主动脉的舒张作用及其可能的作用机制。方法采用离体大鼠胸主动脉张力实验,观察CCE的舒张血管作用,并观察CCE对大鼠胸主动脉产生NO的影响。结果 CCE显著降低苯肾上腺素(PE,10-6mol/L)与高钾(60 mmol/L)预收缩内皮完整血管的张力,对PE诱导血管收缩的抑制作用强于对高钾的作用。但是,CCE对去内皮的主动脉环均无舒张作用;一氧化氮合酶(NOS)抑制剂N-硝基-L-精氨酸甲酯(L-NAME)或吲哚美辛存在的情况下,CCE抑制PE收缩的效应消失。另外,CCE可明显增加大鼠主动脉NO含量。结论CCE对大鼠胸主动脉产生内皮依赖性舒张作用,该作用与促进NO的合成释放有关,也可能与促进前列环素的生成及抑制受体操控性钙通道和(或)肌浆网Ca2+释放有关。
Objective To investigate the vasorelaxant effect of active fraction from Clinopodium chinense（Benth.） O.Ktze（CCE） on isolated thoracic aorta of rats and its mechanism.Methods The isotonic contraction of the thoracic aorta strips in rats was recorded,and the effects of CCE on the contraction of rat thoracic aorta were examined.In addition,thoracic aorta rings were incubated with different concentration of CCE,nitric oxide（NO） level was measured.Results CCE evoked a significant relaxation in endothelium-intact aorta rings precontracted by phenylephrine（PE,10-6mol / L） and KCl（60 mmol / L）.But,the effect on endothelium-intact aortas induced by PE was more effective than that induced by KCl.Additionally,CCE had no significant effect on endothelium-denuded aorta rings precontracted by phenylephrine and KCl.Pretreatment with nitric oxide synthase（NOS） inhibitor Nw-nitro-L-arginine methyl ester（L-NAME）,or indomethacin,vasodilatation effect of CCE was remarkably reduced.Furthermore,thoracic aorta rings pretreated with CCE,NO level was significantly increased.Conclusion These results indicate that CCE possesses the endothelium-dependent relaxation which is probably related with promoting synthesis and release of NO and prostacyclin（PGI2）,as well as the inhibition of receptor-operate Ca^2 ＋ channel and（or） release of Ca^2 ＋ from sarcoplasmic reticulum.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第9期2226-2228,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家基础科学人才培养基金(国家级大学生实践创新训练计划项目)(No.J0630858)
中国药科大学中央高校基本科研业务费专项基金培育项目(No.JKP2009008)
关键词
风轮菜
胸主动脉
血管舒张
一氧化氮
Clinopodium chinense
Thoracic aorta
Vasodilatation
Nitric oxide
Prostacyclin
