摘要
利用染色体荧光原位杂交技术(FISH),将3种重复序列5S rDNA、45S rDNA和C0t-1 DNA用不同荧光物进行标记,对我国10个不同地理来源的苜蓿种质(Medicago sativa L.;2n=4X=32)进行了染色体多态性检测。结果表明,利用以上重复序列可以较好地将苜蓿32条染色体区分为16对特征不同的染色体,10份不同种质材料FISH带纹特征表现高度相似,比较不同种质间同源染色体重复序列杂交特征,揭示出种质群体内和群体间多态性染色体的存在,其中不同的同源染色体多态性表现不尽一致,1号染色体(随体染色体)多态性最高,10份材料中检出7个多态型,3、4、15号染色体保守性较强,在不同种质间表现为单态,其他染色体多态性居中。对在地理分布上自西向东的10个材料进行染色体多态性比较,结果显示分布于西藏、新疆以及分布在辽宁的材料部分染色体多态型显著区别于其他材料。
A cytological investigation was conducted on the 10 Chinese tetraploid alfalfa（Medicago sativa L.;2n=4X=32）germplasm accessions from the different regions using fluorescence in situ hybridization（FISH）.Three repetitive sequences,5S rDNA,45S rDNA,and C0t-1 DNA were adopted as FISH probes.The result showed that 32 chromosomes of alfalfa could be characterized as 16 pairs with the above probe combinations,and the FISH patterns among different materials revealed high similarity.Chromosome polymorphisms were uncovered both within and among genotypes.Chromosome 1（satellite chromosome）with 7 variants showed the highest polymorphism,while 3、4 and 5 homologue chromosomes were more conserved and monomorphic.Chromosome polymorphisms were compared among 10 alfalfa germplasm accessions from western to eastern regions of China.The result showed that a few of chromosome variants in germplasm accessions from Tibet,Xinjiang,and Liaoning were distinct from those in the others.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2012年第5期782-788,共7页
Journal of Plant Genetic Resources
基金
国家高技术研究发展计划(863计划)项目(2009AA10Z108)
关键词
苜蓿
荧光原位杂交
染色体
多态性
Alfalfa
Fluorescence in situ hybridization（FISH）
Chromosome
Polymorphism
