摘要
以陆地棉品系棕彩选1号为材料,利用cDNA-AFLP技术研究棉花低温处理前后基因表达的差异。试验采用64对引物组合进行扩增,共获得2321个条带,从中得到34条表达存在差异的条带。根据表达模式的不同,对其中表达存在差异的22个转录衍生片段(Transcripts derived fragments,TDFs)进行了克隆、测序和序列分析。结果表明:在低温胁迫条件下获得的18条TDFs中,上调表达2条(11%),下调表达9条(50%),另外7条(39%)为瞬时表达;而在恢复2 d后得到了4条特异表达的TDFs。22个TDFs中,21个TDFs与NCBI中已知功能基因存在同源性,这些基因包括假定蛋白、转座子、线粒体、过氧化物酶体增殖物激活受体和逆境胁迫反应关键基因等。另有1个TDFs无同源序列。
The objective of this study was to determine temporal expression profiles of transcripts during cold acclimation of cotton plants using cDNA-AFLP. The upland cotton line, ZongCaiXuan No. 1, was used for expression analysis. After being screened with 64 primer combinations, 2321 bands were amplified and 34 polymorphic transcript-derived fragments（TDFs） were identified. According to differences in gene expression patterns, 22 polymorphic TDFs were isolated and cloned. Among the 22 TDFs, 18 were found to be related with the low temperature treatment, including two with up-regulated expression, nine with down-regulated expression and seven that were transiently expressed. In addition, there were four TDFs expressed specifically after a two-day recovery under normal conditions. An NCBI Blast search showed that 21 TDFs were homologous to functional genes, including a hypothetical protein, a transposon, a peroxisome proliferator activated receptor and stress defense genes, but the function of other one TDF remains unknown.
出处
《棉花学报》
CSCD
北大核心
2012年第5期386-392,共7页
Cotton Science
基金
安徽省教育厅重点项目(KJ2011A103)
安徽省棉花产业创新体系
