摘要
目的:研究壳聚糖(chitosan,CS)修饰的羟基磷灰石(hydroxyapatite,HA)纳米粒载体携带骨形态发生蛋白2(Bone morphogenetic protein2,BMP2)基因转染成骨细胞,及转染后对细胞增殖与分化的影响。方法:采用MTT法检测HA-CS纳米粒载体对成骨细胞的毒性反应;用HA-CS纳米粒载体转染技术,将BMP-2目的基因导入成骨细胞,评估转染效率;检测细胞增殖情况及碱性磷酸酶(alkaline phoaphatase,ALP)活性。结果:MTT结果提示HA-CS纳米粒混悬液浓度在500mg/L以下对成骨细胞无毒性作用;其转染效率优于没经修饰的HA;转染后促进成骨细胞增殖并增加了ALP活性。结论:HA-CS纳米粒在一定浓度下对成骨细胞无毒性作用,作为输送载体可以安全地将目的基因BMP-2导入成骨细胞,并促进了成骨细胞的增殖与分化。
Objective： To observe the impact of transfection of bone morphagenetic protein 2（BMP--2） gene mediated by hydroxyapatite（HA） nanoparticles with chitosan（CS） surface modification on proliferation and differentiation of osteoblast. Methods： We detect the cell toxicity reaction of HA--CS nanoparticles carrier to the osteoblast by MTT assay. The BMP--2 gene were transfected into osteoblast mediated by HA--CS nanoparticles, and the trans- fection efficiency was evaluated. The proliferation of osteoblast and the activity of alkaline phoaphatase（ALP） were measured. Results： MTT assay demonstrated that HA--CS nanoparticles suspension liquid had no significant effects on the growth of osteoblast at the concentration below 500/μg/ml, and transfection efficiency by modified HA was better than not. Osteoblast proliferation and the bone ALP group activity were enhanced after transfected. Conclusion： HA--CS nanoparticles was non--toxic at a certain concentration. And as a carrier, purpose BMP--2 gene could be safely transfected into osteoblast mediated by it, promoting the proliferation and differentiation of osteoblasts.
出处
《口腔医学研究》
CAS
CSCD
2012年第9期857-860,共4页
Journal of Oral Science Research
基金
黑龙江省教育厅科学技术研究项目(编号:13551538)
佳木斯大学青年基金项目(编号:Sq2011-022)
