摘要
目的：探讨肉碱对大鼠心肌线粒体呼吸链功能及抗氧化能力的影响。方法：40只2月龄雄性Wistar大鼠随机均分为4组（n=10）：对照组（C）、补充肉碱组（LC）、运动训练组（T）、运动训练＋补充肉碱组（TLC）。LC和TLC组每天按300 mg/（kg bw.d）剂量灌胃补充肉碱一次,T组和TLC组进行递增负荷水平跑台运动训练,共6周。力竭运动后即刻取样,差速离心法提取线粒体。分光光度法测定线粒体呼吸链CⅠ-Ⅳ、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果：与C组相比,LC组、T组和TLC组CⅠ及CⅣ活性均显著升高（P〈0.05,P〈0.01）,T组和TLC组CⅡ及CⅢ活性均显著升高（P〈0.05,P〈0.01）;与LC组相比,TLC组CⅠ-Ⅳ活性均显著升高（P〈0.05,P〈0.01）;与T组相比,TLC组CⅠ及CⅣ活性均显著升高（P〈0.05）。与C组相比,LC组、T组和TLC组SOD活性均显著升高、MDA含量均显著降低（P〈0.05,P〈0.01）;与LC组和T组相比,TLC组SOD活性均显著升高、MDA含量均显著降低（P〈0.05）。结论：补充肉碱及运动训练均可提高心肌线粒体呼吸链功能及抗氧化能力,从而提高机体的运动能力,且肉碱与运动训练具有协同作用。
Objective： To investigate the effect of exogenous carnitine on function of respiratory chain and antioxidant capacity in mitochondria of myocardium in rats.Methods： Forty male Wistar rats were randomized to 4 groups（n=10）： static control（C）,supplementation of carnitine（LC）,exercise-training（T） and training with supplementation of carnition（TLC）.LC and TLC animals were perfused carnitine by the dose of 300 mg/kg bw·d.T and TLC animals were forced to performed 6-week treadmill training.Heart were prepared immediately after exhaustive running.Myocardium mitochondria was extracted by differential centrifugation.Spectrophotometric analysis was used to evaluate activities of respiratory chain complex（C） Ⅰ-Ⅳ and superoxide dismutase（SOD）,malondialdehyde（MDA） level in myocardium mitochondria.Results： To compare with C group,CⅠand CⅣ activity in LC,T and TLC group were increased significantly（P〈0.05,P〈0.01）,CⅡ and CⅢ activity in T and TLC group were increased significantly（P〈0.05,P〈0.01）;to compare with LC group,CⅠ~Ⅳ activity in TLC group were increased significantly（P〈0.05,P〈0.01）;to compare with T group,CⅠand CⅣ activity in TLC group increased significantly（P〈0.05）.To compare with C group,SOD activity increased remarkably,MDA was remarkably lower（P〈0.05,P〈0.01） in LC,T and TLC group;To compare with LC and T group,SOD activity increased remarkably,MDA was remarkably lower（P〈0.05） in TLC group.Conclusion： Carnitine and training could improve function of respiratory chain and increased antioxidant capacity in myocardium mitochondria,there was better function of cooperation between carnitine and training.
出处
《中国应用生理学杂志》
CAS
CSCD
2012年第5期405-409,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(31060145)
甘肃省自然科学基金资助项目(1107RJZA087)
关键词
肉碱
心肌
线粒体
呼吸链
酶复合体
超氧化物歧化酶
丙二醛
carnitine
myoeardium
mitochondria
respiratory chain
multi-enzyrmatic complexes
superoxide dismutase
malondialdehyde
