摘要
旨在筛选和分析内蒙古绒山羊与毛和绒生长相关的差异基因,本研究采用mRNA差异显示技术(DDRT-PCR)研究内蒙古绒山羊胎儿期不同日龄皮肤中控制初级毛囊和次级毛囊不同分化的差异表达序列标签。结果,共筛选了15条差异表达序列标签(dbEST登录号JK711022-JK711036),其中3条ESTs与毛囊发育有关,5条ES-Ts与次级毛囊和皮脂腺的发育有关。生物信息分析显示,JK711036与甲状腺激素受体相互作用蛋白12(TRIP12)基因有关,其他ESTs功能未知。结果表明,胎儿期初级毛囊和次级毛囊在多种代谢途径上都存在差异表达的基因,这将为进一步研究山羊毛和绒的形成分子机制奠定基础。
To screen and analyze the genes associated with growth of goats＇ wool and cashmere, differential display reverse transcription polymerase chain reaction （DDRT-PCR） technology was used to study the differentially expressed sequence tags （ ESTs ） at different embryo ages in skin primary and secondary follicles of Inner Mongolia Cashmere goats. 15 ESTs were identified （Acc _N： JK711022-JK711036） , of which 3 ESTs might play a role in the development of hair follicle, and 5 ESTs might be related to secondary follicles and sebaceous glands. The results of bioinfor- matics analysis showed that JK711036 shared homology with other species＇ TRIP12 gene, but the function of other ESTs were unknown. All these were showed that many differential expressed genes was associated with primary and secondary wool follicles on metabolism pathways, which would establish molecular foundation for the formation of wool and cashmere in goats.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第9期1499-1503,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家转基因项目(2008zx08008-002)
博士后基金资助
