摘要
目的采用人红细胞为实验模型,探索槲皮素对溶菌酶淀粉样纤维细胞毒性的抑制作用。方法制备溶菌酶淀粉样纤维,在溶菌酶纤维溶液中加入槲皮素,用原子力显微镜观察槲皮素对淀粉样纤维的分解作用;在红细胞悬液中加入溶菌酶淀粉样纤维和槲皮素,用扫描电镜观察细胞形态;SDS凝胶电泳分离细胞膜蛋白,检测在槲皮素存在的条件下,溶菌酶纤维诱导膜蛋白聚集的作用。结果槲皮素能够破坏淀粉样纤维结构,使纤维解聚,从而使溶菌酶淀粉样纤维的细胞损害作用降低,包括抑制溶菌酶纤维诱导的细胞聚集和细胞膜蛋白交联。结论槲皮素能够破坏成熟的溶菌酶淀粉样纤维结构,抑制淀粉样纤维对细胞膜的损害作用。槲皮素的这种作用与其分子的疏水性和抗氧化作用有关。
Objective To investigate the inhibitory effect of quercetin on cytotoxicity of amyloid fibrils by using human erythrocyte as an in vitro model. Methods Lysozyme fibrils were prepared and used in the experiments. The disruptive effect of quercetin on lyso-zyme fibrils was observed under atomic force microscopy. Morphology of erythrocytes treated with lysozyme fibrils and quercetin was imaged by scanning electron microscopy. SDS-PAGE was used to analyze the protein pattern of erythrocyte membrane incuba-ted with or without the fibrils and quercetin. Results The results showed that quercetin played a disruptive role on lysozyme fibrils, transforming the fibrils to disaggregated species. As a consequence,the fibrillar toxicity to erythrocytes was attenuated. The cyto-toxicity of lysozyme fibrils included inducing aggregation of the cells and triggering cross-linking of membrane proteins. Conclusion Quercetin is able to disrupt amyloid structure and to inhibit the fibril-induced cytotoxicity. The fibril-disrupting effect of quercetin is possibly related to both its anti-oxidative potency and molecular hydrophobicity.
出处
《西北药学杂志》
CAS
2012年第5期444-447,共4页
Northwest Pharmaceutical Journal
