摘要
目的 探讨二乙基二硫代氨基甲酸钠 (DDC)对耐药胃癌细胞的作用及可能的机理。方法 应用MTT方法 ,化学发光法测定SOD活性以及流式细胞仪检测细胞内药物浓度。结果 DDC与阿霉素 (adriamycin ,adr)共同应用可明显抑制耐药细胞及药敏肿瘤细胞的生长。在DDC组 ,adr对SGC790 1/WT的半数生长的抑制剂量 (IC5 0 )为 0 79μg/mL。在同一组中 ,adr对SGC790 1/VCR的IC5 0为 0 3 2 μg/mL。流式细胞术证实 ,耐药细胞中的药物蓄积量为药敏细胞中的1/2 ,SOD活性检测表明 ,DDC明显抑制了SOD活性。
Aim To evaluate the effect and mechanism of Diethyldithiocarbamate (DDC) to drug-resistent gastric cancer cell.Method MTT assay was used to evaluate the inhibitory effect of DDC on the growth and on the multidrug resistance (MDR) of gastric tumor cell.The activation of SOD was detected by chemilumicescence,and the drug accumulation by flow cytometry.Result Our results implied that:DDC could largely decrease the usage of adriamycin (adr) in the treatment.In the DDC group,the IC50 values of adr for SGC7901/WT and SGC7901/VCR were found to be 0.79μg/mL and 0.32 μg/mL,respectively.The drug accumulation in MDR cell line was half of the parent cell lineDDC inhibited the activation of SOD markedly.Conclusion The activation of SOD may has a fairly critical role in the reversal of multidrug-resistant cancer cell,and that the action of DDC has important value in cancer therapy.
出处
《胃肠病学和肝病学杂志》
CAS
2000年第1期27-30,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金课题!第39525020及39880007号资助
关键词
多药耐药
逆转作用
胃癌细胞
DDC
Diethyldithiocarbamate MDR Reversal Gastric cancer cell
