摘要
目的研究汞在神经组织的原位沉积部位，为理解和阐明汞的毒理和汞中毒症状提供有价值的线索。方法雄性小鼠4只暴露于金属汞蒸气中，汞蒸气浓度为5mg／m3，暴露时间为每周6d，每日30min，共4周。显示组织中汞沉积部位的方法为醋酸银金属自显影方法。结果汞颗粒主要分布于大脑皮层的Ⅲ、Ⅳ、Ⅴ层以及大脑深部核团的神经元胞质中，其余区域分布较少或无。在脑干，汞颗粒主要分布于神经核团的大、中型细胞胞质中，其余区域分布少。在小脑，粗大的汞颗粒主要分布于浦肯野细胞层，其余细胞层可见少量散在分布。粗大汞颗粒主要分布于脊髓（C1）的Ⅶ～Ⅹ层大型运动神经元胞质中，而在Ⅰ～Ⅵ层汞颗粒分布小而少。结论汞金属在大脑皮层Ⅲ、Ⅳ、Ⅴ层的沉积，可能是汞引起神经行为功能改变的病理基础；汞在大脑基底节及小脑的沉积可能与汞中毒的神经系统症状之一──震颤有关。
Objective To study the location of mercury in the neural tissue and to give cite in understanding the principle and clinical features of mercurialism. Methods The autometallographical technique was used to determine the distribution and cellular localization of mercury deposits in tha mice central nervous system after exposure to elemental mercury vapour (5 mg/m3 for 4 weeks)30 min a day for 6 days/week. Results In cerebral sections from the mice exposed to Hg0(5 mg/m3), the staining intensity in the corticall cells varied among layers,being greatest in laminae Ⅲ,Ⅳ and Ⅴ,and in other laminae containing little or no. In the cerebella, mercury deposits were limited to the Purkinje cells, while heavy deposits of mercury were present in the deep nuclei of the brainstem. Mercury deposits could be observed in the spinal cord neurons located in Rexed laminae Ⅶ～Ⅹ, while in Rexed laminae Ⅰ~Ⅵ there coule be light or little mercury deposits. The main target cells were the neurons, but glia the cells also contained scattered mercury deposits. Conclusions Mercury deposits in the cerebral cortex were associated with neurobehavioral changes caused by mercury. Mercury deposits in the cerebral hasal ganglia and cerebella might be responsible for tremor caused by exposure to elemental mercury vapour.
出处
《中华神经科杂志》
CAS
CSCD
2000年第2期110-111,共2页
Chinese Journal of Neurology
关键词
汞中毒
中枢神经系统
汞颗粒
沉积
Mercury poisoning
Silver acetate autometallography
Central nervous system
