摘要
目的 :探讨检测AML1 ETO融合转录本在t( 8;2 1)急性粒细胞白血病M 2型 [M 2 /t( 8;2 1]的临床诊断和预后判断中的意义。方法 :用半筑巢式逆转录聚合酶链反应 (RT/PCR)技术检测AML1/ETO融合基因转录本 ,应用Southernblot技术及限制性内切酶技术检测PCR产物的特异性。结果 :在 19例AML M 2中共检出 10例存在AML1/ETO融合基因转录本 ,阳性率为 5 2 .6 % ,2例MDS中 1例为阳性 ,其余病例包括 3例M4均为阴性 ,第一轮PCR的检测水平为 1/ 10 3 10 4 个细胞 ,第二轮PCR的敏感性达 1/ 10 4 10 5个细胞 ,说明该技术的敏感性很高。结论 :半筑巢式RT/PCR技术是一种快速、简便、灵敏、可靠的检测AML1/ETO融合转录本的方法 ,对M2 /t( 8;2 1)
Objective:To study the clinical significance of detection of AML1/ETO fusion gene in diagnosis and prognosis for t(8;21) acute myelogenous leukemia M2(M2/t(8;21).Methods:AML1ETO fusion transcripts were detected by reversetranscriptase chain reaction (RT/PCR).The specificity of the PCR products were assured by a restriction digest with Taq I and hybridization with an AML1 cDNA probe C6E6H2 labeled with α32P.Results:AML1ETO fusion transcripts were detected in 10 out of 19 patients with AMLM2 phenotype.The positive rate was 52.6%.One of the two patients with MDS had AML1ETO fusion trancripts.The results were negative in the rest of patients including three cases of AMLM4.The level detected from the first step of RT/PCR was one in 10104 cells,while the level from the second step was one in 105?106 cells.These data suggested that the technique was extremely sensitive.Conclusion:The seminested RT/PCR was a simple,rapid,accurate and sensitive approach for detection of AML1ETO fusion transcripts and appeared to be of value in diagnosis and predicting the prodictig the prognosis of M2/t(8;21).
出处
《中国优生与遗传杂志》
2000年第2期18-20,共3页
Chinese Journal of Birth Health & Heredity
关键词
急性白血病
融合转录本
AML1/ETO
PCR
Leukemia
myelogenous
AML
Fusion transcript
AML1-ETO
Polymerase chain reaction
