摘要
为探讨荒漠植物牛心朴子(Cynanchum komarovii)响应干旱胁迫的渗透调节机制,采用不同浓度(0～25%)的聚乙二醇(PEG-6000)对愈伤组织进行模拟干旱胁迫,检测了不同程度干旱胁迫下牛心朴子中渗透调节物质K+、Na+、脯氨酸、甜菜碱和可溶性糖(果糖、蔗糖和海藻糖)的含量。结果显示,与对照组相比,处理组牛心朴子的脯氨酸和可溶性糖含量显著升高(P<0.05),且具有浓度和时间依赖效应。在可溶性糖中,果糖和蔗糖只在高浓度处理组有所增加,而海藻糖含量在所有处理组均明显升高(P<0.05),且增幅较大(10%PEG浓度组为31%,25%PEG浓度组为98%),同时海藻糖含量与PEG处理浓度的相关系数最大(R2=0.9437,P<0.01)。以上研究结果表明,海藻糖是牛心朴子响应干旱胁迫主要的渗透调节物质。
Cynanchum komarovii is one of the desert plants with drought resistance. To study its osmotic adjustment mechanism under drought stress, the calluses of Cynanchum Komarovii were treated with PEG-6000 at different concentration （0 -25%）, and then content of the osmotic adjustment substances （K＋ , Na＋ , proline, betaine, soluble sugars and so on） were measured. Results showed that with the rise of PEG-6000 concentration, the contents of proline and soluble sugars increased obviously （P〈0.05）. Under drought stress, the contents of fructose and sucrose increased at high concentration PEG-6000 treatments, while the content of trehalose increased very obviously at all PEG-6000 treatments, with 31% enhancement at 10% PEG-6000 treatment and 98% at 25 % PEG-6000 treatment, respectively. The correlation analysis showed that the coefficient between trehalose content and PEG-6000 concentration was the highest with R2 =0. 9437 （P〈0.01）. These results suggest that trehalose is the primary osmotic adjustment substance of Cynanchum komarovii resisting to drought stress.
出处
《中国沙漠》
CSCD
北大核心
2012年第5期1275-1282,共8页
Journal of Desert Research
基金
国家自然科学基金项目(40825001
40930636)
中国科学院寒区旱区环境与工程研究所青年人才成长基金项目(Y084921001)共同资助
关键词
牛心朴子
渗透调节
海藻糖
果糖
蔗糖
Cynanchum komarovii
osmotic adjustment
trehalose
fructose
sucrose
