摘要
【目的】为了给梨转基因研究提供稳定、高效的离体再生体系,【方法】以‘苹果梨’为试材,NN69作为基本培养基,研究了植物生长调节剂种类、暗培养时间及外植体类型等因素对其再生体系建立的影响,筛选出较适合‘苹果梨’不定芽分化的培养条件。【结果】结果表明,较适合‘苹果梨’分化的外植体为继代4次以上(含4次)的组培苗叶片,适宜分化的培养基为NN69+1.5 mg.L-1TDZ+0.4 mg.L-1NAA,结合20 d的暗培养,再生频率达到71.00%,平均再生芽数为2.21。【结论】影响‘苹果梨’离体叶片再生的最关键因素是外植体的继代次数。
[Objective]In order to establish a stable and effective regeneration system for pear genetic transformation, [Method] the effects of plant growth regulators, dark culture time and the explant types on the adventitious bud regeneration of Pingguoli pear （Pyrus breschneideri Rehd） leaves in vitro were studied. [Result]The results showed that the best explants for Pingguoli regeneration were the leaves which were from plants subcultured more than 4 generations, and the optimum medium was NN69 ＋1.5 mg·L^（-1） TDZ ＋ 0.4 mg·L^（-1） NAA. After 20 d dark culture, the regeneration frequency was 71% and the bud number per explant in average was 2.21. [Condusion]The studies suggested that the key factor which influences the adventitious bud regeneration of Pingguoli pearleaves in vitro is the age of subculture.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第5期800-803,F0003,共5页
Journal of Fruit Science
基金
国家自然科学基金(30960231)
关键词
'苹果梨’
再生
不定芽
‘Pingguoli pear＇ （Pyrus breschneideri Rehd）
Regeneration
Adventitious buds
