摘要
从水稻基因组DNA中扩增出1 184bp的Ospgip1基因片段。该片段包含930bp的完整开放阅读框,终止密码子为TAA,无内含子。RT-PCR结果表明,Ospgip1基因在水稻抗、感纹枯病品种中均能表达,但不同生育期、不同部位表达量有差异。实时PCR结果显示,抗病品种YSBR1和中感品种Jasmine 85中Ospgip1的表达量要明显高于感病品种Lemont;稻苗黄化处理后,无论是抗病品种还是感病品种的Ospgip1表达量均显著提高;纹枯病菌侵染使得抗病品种Ospgip1表达量大大增加,而对感病品种影响不大。
A fragment of 1 184 bp of the Ospgipl gene including a complete open reading frame （OFR） of 930 bp had been amplified, which had the termination codon of TAA and no intron, RT-PCR results showed that the Ospgipl gene could be expressed in all the rice cultivars while their expression levels differed among growth stages or organs. Real-time PCR results indicated that the expression levels in resistant cuhivars YSBR1 and Jasmine 85 were significantly higher than that in the susceptible cuhivar Lemont. The expression level was also upregulated in the etiolated seedlings, Inoculation with the pathogen of rice sheath blight significantly upregulated the expression level of the Ospgipl gene in resistant cultivars while exerted no impact on the expression level of the gene in the susceptible one. Key words： expression characteristics; rice cultivar; Ospgipl gene; resistance
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第5期629-632,共4页
Chinese Journal of Rice Science
基金
国家转基因生物新品种培育重大专项(2009ZX08001-014B)
江苏省自然科学基金资助项目(BK2010305)
