摘要
通过实时定量PCR分析了稻曲病菌中5个传统内参基因18SrRNA、GAPDH、ubiquitin、β-actin、α-tubulin的mRNA差异表达情况,并利用geNorm软件分析了它们在4个发育时期和NaCl胁迫处理中的表达稳定性。结果表明,在菌龄为7d、8d、9d、10d的稻曲病菌中,α-tubulin-2和β-actin表达稳定;在0.4mol/L NaCl溶液处理0h、4h、8h、12h、16h后,β-actin和α-tubulin-2表达稳定。因此,当利用荧光定量PCR分析比较稻曲病菌基因表达差异时,可选择α-tubulin和β-actin作为内参基因。
A total of five traditional reference genes of Ustilaginoidea virens were systematically compared by using real-time quantitative PCR, including 18S rRNA, GAPDH, ubiquitin, ffactin and octubulin. The expression stability of the 5 candidate reference genes was evaluated with geNorm program when the fungus was 7, 8, 9 and 10 days old or exposed to 0.4 mol/L NaC1 for 0 h,4 h, 8 h, 12 h, 16 h. The results showed that octubulin-2 and ffactin were stably expressed when the fungus was at different days of age. For the case of 0.4 mol/L NaC1 stress, ffactin and cctubulin-2 were also expressed stably. As a conclusion, ffactin and octubulin could be used as the reference genes for normalization of real-time quantitative PCR data.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第5期615-618,共4页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31171519
31101130)
浙江省自然科学基金资助项目(LY12CD6001)
