摘要
本文对透明颤菌血红蛋白基因 (vgb)和λ噬菌体裂解基因 (S RRz)在不同宿主大肠杆菌中的外源表达及其在聚β 羟基丁酸酯 (PHB)生产中的应用进行了研究。实验结果表明 ,同时携带vgb、S RRz和 phbCAB三种基因的产PHB基因工程菌VG1 ( pTU1 4) ,经过 82h的摇瓶补料分批培养 ,菌体浓度可以高达 2 5 9g/L ,PHB百分含量则可在 52h时达到 95%以上 ;此外 ,该菌株不仅可以实现摇瓶高密度发酵培养和PHB产品的大量积累 ,还可以同时实现菌体细胞的可诱导裂解破壁 ,因此是一株具有潜在工业应用价值的多功能PHB生产菌株。
Cloning and expression of Vitreoscilla hemoglobin gene ( vgb ) and λ phage lytic genes ( S RRz ) in production of Poly β hydroxybutyrate(PHB) was studied in different Escherichia coli hosts such as E.coli JM105, E.coli JM109 and VG1. In the recombinant strains, VG1(pTU14) was a superior one which simultaneously contained three exogenes including vgb , S RRz and PHB biosynthesis genes( phb CAB). The experimental results showed that after 82 hours fed batch cult ure in LBG medium in shaking flask, cell concentration of VG1(pTU14) could reach 25.9g/L, which was the highest PHB production ever reported, and till 52h PHB content could be higher than 95%. Additionally, inducible cell lysis was also attained successfully in recombinant VG1(pTU14) accompanying with its high cell density culture. Therefore, VG1(pTU14) is a novel potential strain with promising prospect in industrial production of PHB.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第3期284-289,共6页
Acta Microbiologica Sinica
基金
国家"九五"攻关项目 !( 96 C0 3 0 3 0 2 )
国家自然科学基金!重点项目 ( 2 983 4 1 0 3 )
国家自然科学基金面上资助!项目
关键词
VHB基因
S-RRZ
聚Β-羟基丁酸酯
高密度发酵
Vitreoscilla hemoglobin gene ( vgb) , λ phage lytic genes( S RRz ), Poly β hydroxybutyrate(PHB), High cell density culture, Inducible cell lysis
