摘要
以猪瘟病毒中国兔化弱毒疫苗株 (CSFVC株 )为实验材料 ,研究该病毒株在原代牛睾丸细胞中增殖的基本特性与规律。找到了一株能够使用免疫荧光技术进行检测的C株猪瘟病毒 ,并对病毒滴度的测定方法进行了改进 ,发展完善了荧光斑技术 (F PFU)。使用改进的病毒滴度测定方法 ,对接毒后培养上清中病毒滴度的变化趋势进行检测。在原代牛睾丸细胞中 ,接毒后 5d ,几乎所有的细胞都可被病毒感染 ;释放到培养液中有活性的病毒粒子达到 10 5F PFU/mL以上 ,后维持在该水平。对原代细胞中细胞种类随代次增加的变化趋势进行了观察 ,并对CSFV的增殖特点及生产中出现的一收毒价不稳定、多次收获病毒等现象的机制进行探讨。并对影响猪瘟病毒增殖的因素进行了实验 ,不仅加深了对猪瘟病毒C株在原代牛睾丸细胞中增殖规律的了解 。
Some multiplication properties of the Lapinized Chinese strain of Classical Swine Fever Virus (CSFV C strain) in primary bovine testicular cells were studied. One CSFV C strain which can be detected by immunofluorescence technique was found and a new method in titering called flurescent plaque formation unit (F PFU) was developed. It took about 5 days for all cells of primary bovine testis to be infected by CSFV and the titre of progeny virions in medium was 10 5 F PFU/ml from then on. The cell types of cultured bovine testicular cells changed along with cell passages. The mechanism of some phenomena in vaccine production was showed and some factors which effect the multiplication of CSFV C strain in primary bovine testicular cells were studied. Aknowledgements on the multiplication of CSFV C strain in primary bovine testicular cells were enriched and a new way to raise the titer of CSFV was suggested.
出处
《中国病毒学》
CSCD
2000年第2期170-179,共10页
Virologica Sinica
基金
国家攀登计划资助项目 !(85 - 4 4 - 0 2 - 0 5)
关键词
猪瘟病毒
弱毒疫苗
C株
病毒增殖
原代细胞
Classical Swine Fever Virus (CSFV)
The Lapinized Chinese strain of CSFV (CSFV C strain)
Immunofluorescence technique
Flurescent plaque formation unit (F-PFU)
Vaccine production
