摘要
CUEDC2(CUE domain containing protein 2)是本实验室发现的功能未知蛋白质。已有研究证明CUEDC2通过抑制孕激素受体PR影响乳腺癌细胞的生长,同时CUEDC2的高表达能够降解雌激素受体ER而导致乳腺癌内分泌治疗耐药。为了深入研究CUEDC2在乳腺癌中的功能,构建了稳定过表达CUEDC2的MCF-10A细胞系。利用逆转录病毒将非融合形式的绿色荧光蛋白质GFP和CUEDC2基因稳定整合至靶细胞的基因组中,经过流式分选获得GFP阳性细胞,即为稳定表达CUEDC2的细胞株。上述方法高效快捷,极大提高了稳定细胞株的构建效率。同时该稳定细胞株的建立为CUEDC2的功能研究提供了必要的工具。
CUEDC2 （CUE domain containing protein 2） is a functionally unknown protein. In previous work, CUEDC2 interacts with the progesterone receptor （PR） and promotes progesterone-induced proteasomal degradation of PR. Further study suggested that CUEDC2 is a crucial determinant of resistance to endocrine therapies in breast cancer and over-expressed in many tumors. In order to address the question if CUEDC2 affect the proliferation or migration of breast cancer ceils, a stable cell line is constructed with the normal glandular epithelium cell, MCF-10A, by retrovirus. Cells with GFP expression indicating the expression of CUEDC2, thus, the GFP positive cells were selected by fluorescence-activated cell sorting. By combining the retrovirus infection and cell sorting, the selecting efficiency was markedly increased. This cell line will largely benefit the study of CUEDC2 in breast cancer.
出处
《科学技术与工程》
北大核心
2012年第24期6148-6151,6162,共5页
Science Technology and Engineering
基金
国家自然科学基金(81172008)资助
