摘要
目的基质金属蛋白酶9基因多态性与慢性阻塞性肺疾病（COPD）易感性的关系。方法应用聚合酶链反应一限制性片段长度多态性法（PCR—RFLP）检测69例COPD患者，61例健康者基质金属蛋白酶9R279Q位点等位基因和基因型。结果①COPD患者基质金属蛋白酶9R279Q位点突变型（QQ）及杂合型（R／Q）基因型频率为31．8％和14．6％，健康对照组分别为37．8％和26．2％，两组基因分布频率差异无统计学意义；而野生型（RR）在两组中分布频率分别为53．6％和36％，其分布频率差异有统计学意义（P〈0．05）；在COPD组和健康对照组中R和Q等位基因频率差异有统计学意义（P〈0．05）。②在吸烟者中，QQ及R／Q基因型在两组中分布差异无统计学意义；而RR基因型在两组中分布差异有统计学意义（P〈0．05）。不吸烟者中，QQ、R／Q、RR基因型在分布差异均无统计学意义。吸烟者中，R和Q等位基因频率在两组中分布差异有统计学意义（P〈0．05）；在不吸烟者中，R和Q等位基因频率在两组中分布差异无统计学意义。结论①MMP-9R279Q基因多态性可能是COPD发病的危险因素。②R等位基因可能为吸烟者患COPD的一个易感因素。
Objective To investigate the correlation between matrix metalloproteinase 9 （MMP-9） gene polymorphism and the susceptibility of chronic obstructive pulmonary disease（COPD）. Methods Examine the frequency of the genetype of MMP-9 in 69 patients and 61 control subjects by reslriction fragment length polymorphism. Results ①The frequency of MMP-9 QQ, R/Q genotype were 31.8%, 14.6% in COPD group, respectively, and 37.8%, 26.2% in control group, respectively. There were no significant differences between the two groups （ P 〉0.05）. The distribution frequencies of RR genotypes were significantly different between two groups （ P 〈0.05）. The differences in allele frequencies were also significant between the two groups （ P 〈0.05）. ②Smokers, there were no significant differences on QQ, R/Q genotypes in COPD group and in controls. There were significantly different on RR genotypes in COPD group and in controls （ P G0.05）. In non-smokers, there were no significant differences on QQ, R/Q,RR genotypes in two group. Smokers, there were significantly different on the frequencies allele of R and Q between two groups. In non-smokers, there were no significant differences on the frequencies allele of R and Q between two groups. Conclusions ① MMP-9 R279Q polymorphisms maybe a genetic risk factor for COPD. ②The allele gene of R maybe a susceptibility factor for COPD in smokers.
出处
《国际呼吸杂志》
2012年第16期1214-1217,共4页
International Journal of Respiration
关键词
基质金属蛋白酶
慢性阻塞性肺疾病
基因多态性
吸烟
Matrix metalloproteinases
Chronic obstructive pulmonary disease
Polymorphism Smoking
