摘要
为研究猪源脑心肌炎病毒(EMCV)GXLC株对小鼠的致病性,将其接种4周龄健康BALB/c小鼠,观察临床症状,检查病理变化,测定心重/体重比值,检测心脏、脑、脾脏组织及血清中病毒含量,检测心脏、脑、脾脏组织中细胞因子IL-1β、TNF-α、IL-6 mRNA表达水平及血清中IL-1β、TNF-α蛋白含量。结果显示,感染小鼠出现明显的临床症状,感染后第4天开始死亡,第5～7天死亡达到高峰;心脏、脑出现典型的大体病理变化和组织病理学变化,心重/体重比值显著升高、组织病理学炎症分值显著增加;感染后第1天即可在心脏、脑、脾脏及血清中检测到大量病毒,直至第14天仍能检测到病毒;血清中IL-1β、TNF-α蛋白含量显著升高,心脏、脑、脾脏组织中IL-1β、TNF-α、IL-6 mRNA表达水平显著上调,而且细胞因子表达的高峰期与出现明显临床症状、严重病理损害及死亡高峰期密切相关。结果表明,猪源EMCV GXLC株对小鼠具有高度致病性,IL-1β、TNF-α、IL-6等促炎细胞因子在EMCV对小鼠的致病机制中发挥重要作用。
The porcine EMCV GXLC strain was used to infect 4-week-old healthy BALB/c mice to evaluate its pathogenicity.After infection,the clinical signs were observed;the pathological changes were analyzed and the ratios of heart weight to body weight were recorded;the viral loads in heart,brain,spleen and serum were determined;the protein loads of IL-1β and TNF-α in serum and the mRNA expression levels of IL-1β,TNF-α and IL-6 in heart,brain and spleen were detected.The results showed that the infected mice displayed obvious manifestations,and began to die on 4 days post-infection（DPI）,reaching the death peak from 5 to 7 DPI.The heart and brain of the infected mice showed apparent gross lesions and histopathological changes,of which the ratios of heart weight to body weight and the histopathological scores were significantly increased.A large number of viruses were detected in brain,heart,spleen and serum from 1 DPI up to 14 DPI.The protein loads of IL-1β and TNF-α in serum from the infected mice were significantly increased and the mRNA expression levels of IL-1β,TNF-α and IL-6 in heart,brain and spleen were significant up-regulation,with closely positive correlation in time with obvious manifestations,severe pathological lesions and death peak of the infected mice.The results indicated that porcine EMCV GXLC strain was highly pathogenic to mice and the proinflammatory cytokines IL-1β,TNF-α and IL-6 played important roles in the pathogenesis of EMCV.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第8期179-185,共7页
China Animal Husbandry & Veterinary Medicine
基金
广西科学基金项目(桂科青0728047)
关键词
猪源脑心肌炎病毒
致病性
促炎细胞因子
小鼠
porcine encephalomyocarditis virus
pathogenicity
proinflammatory cytokine
mouse
