摘要
对 12个优良玉米自交系的DNA进行了RAPD分析 ,共筛选了 2 5 0个Operon引物 ,其中 12个引物共扩增出 5 9个多态性产物 ,根据这 5 9个多态性RAPD产物 ,对 12个自交系进行了聚类分析 ,把它们分为 3个群 ,结果与根据系谱法的聚类结果一致 .经过优选 ,用OPN - 11扩增出的 11条DNA带 ,建立了这 12个自交系的RAPD -DNA指纹图谱 .在该图谱中每个自交系都具有特异的DNA指纹 ,可以与其它自交系相区别 .然后 ,用 1和 0分别表示各条DNA带的出现和缺失 ,建立了这 12个自交系的计算机化的DNA指纹 ,可用于玉米自交系的真伪及纯度鉴定 .图 4表 1参
elite maize inbred lines were examined by means of RAPD analysis. Among the 250 Operon primers screened, 12 gave reproducible, polymorphic DNA amplification patterns. 11 bands amplified by primer OPN 11 were used in the development of RAPD DNA fingerprinting of the 12 inbred lines. Each inbred line has its unique fingerprinting and can be easily distinguished from each other. The RAPD results were scored based on the presence or absence of each band and then converted to computer language with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. The RAPD DNA fingerprinting analysis developed by this research can be used in practical seed identification of the 12 elite maize inbred lines, for both true and false test as well as purity determination. Fig 4, Tab 1, Ref 6
出处
《应用与环境生物学报》
CAS
CSCD
2000年第3期223-226,共4页
Chinese Journal of Applied and Environmental Biology
