摘要
以“绿草履虫 -小球藻”共生系统为材料 ,选用 2 0种随机引物对光培养下含小球藻共生体的草履虫和暗培养诱导除去小球藻共生体的草履虫基因组DNA进行遗传多态分析 ,由扩增产物电泳图谱显示 ,用S40 1、S147、S786、S75 9、S86、S15 5、S140等随机引物扩增出 86 6、 1393、 16 37bp等具有相同特征的电泳条带 ;随机引物S174、S75 0、S76 9、S75 5、S10 3等则扩增出 1385、 185 2、 15 79bp等无共生藻草履虫的特有条带。据此推测 ,绿草履虫中的共生小球藻会导致宿主草履虫基因组结构的改变。同时显示小球藻共生体的基因组可能与宿主基因组有某些相似之处。
Paramecium bursaria Chlorella sp.” system,a kind of ideal symbiotic system,was selected to research host symbiont interaction.In order to discover the difference of genomic DNA of host and symbiont,genetic variation of the chlorellae bearing and chlorellae free Paramecium bursaria was detected by randomly amplified polymorphic DNA (RAPD) markers.Genetic variation from 12 polymorphic fragments amplified with primers S174、S750、S769、S755、S103 in both strains of Paramecium bursaria exited,though they shared the same fragments amplified with primers S401、S147、S786、S759、S086、S763、S155.It is implied that some changes in genomic DNA of P.bursaria have occurred owing to symbiotic functions.Also,it is shown that there are the similarity between the amplified product of P.bursaria and symbiotic chlorellae.
基金
国家自然科学基金! ( 3 9970 0 88)
高等学校博士学科点专项科研基金
