摘要
为提高磷素利用率,从花生根际土壤样品中筛选到一株溶磷能力强的菌株B1-A,结合菌落形态特征和ITS rDNA序列分析将其鉴定为黑曲霉(Aspergillus niger)。菌株B1-A的不同接种量(1%,3%,5%)在两种难溶性磷酸盐(FePO4,AlPO4)培养液的溶磷量和pH随时间动态变化表明溶磷量均与pH呈显著负相关,B1-A的溶磷量与接种量、培养时间、磷矿粉浓度有关,实验结果表明菌株B1-A在接种量为3%～5%、培养时间168 h、云南磷矿粉浓度为3 g L-1、江西磷矿粉浓度为5 g L-1时溶磷效果较好。菌株B1-A对磷酸三钙、磷酸铝、磷酸铁、磷矿粉有较强的溶解能力,最高溶磷量分别为418.7、942.3、242.2、177.4 mg L-1,有望为开发高效红壤微生物磷肥提供种质资源。
To improve use efficiency of phosphorus in farming,a phosphate-solubilizing strain of fungi （PSF） labeled as B1-A was isolated from the rhizosphere of peanuts growing in red soil and identified as Aspergillus niger according to its colonial morphological characteristics and ITS rDNA sequence analysis. In the culture prepared out of two hard-to-dissolve phosphates （ FePQ and A1PO, ） ,B1-A was added at a rate of 1% ,3% and 5% separately. It was found that the P dissolu- tion rate in and pH of the culture varied with the time,that a significant negative correlation existed between the rate and the pH,and that the former was also related to B1-A inoculation rate,incubation time and the concentration of rock phos- phate powder （RPP） in the solution as well. Results show that B1-A demonstrated its highest capability when the solution was 3 g L-l in Yunnan and 5 g L^-1in Jiangxi RPP concentration, inoculated with 3 % -5 % of B1-A, and incubated for 168 hours. B1-A was quite good at dissolving tricalium phosphate, aluminium phosphate, ferric phosphate and RPP, and might dissolve as much as 418.7,942.3,242.2 and 177.4 mg L^-1 ,respectively. So B1-A is a promise microbial resource for de- velopment of high efficiency biological phosphorus fertilizer for use in latosolic red soil.
出处
《土壤学报》
CAS
CSCD
北大核心
2012年第5期988-995,共8页
Acta Pedologica Sinica
基金
国家科技支撑项目(2009BADC6B04
2009BADC6B003-3)
国家自然科学基金项目(40871125
30971869)资助
关键词
溶磷真菌
溶磷量
接种量
红壤
Phosphate-solubilizing bacteria
P dissolution rate
Inoculation rate
Red soil
