摘要
目的:探讨核孔蛋白复合体蛋白(Nup88)和PINCH mRNA在结直肠癌组织和肿瘤细胞株中的表达水平及两者的类系。方法:利用RT-PCR的方法研究Nup88和PINCH mRNA在47例结直肠癌组织、44例癌旁无瘤黏膜、45例近端、44例远端黏膜及6个结肠癌细胞株中的表达水平,用Spearman法对Nup88和PINCH进行相关性分析。结果:Nup88和PINCH mRNA在结直肠癌组织中的表达水平高于癌旁无瘤黏膜、近端及远端黏膜,且两者在上述4组标本中表达水平均呈正相关(rs分别为0.31、0.35、0.46及0.48)。结论:Nup88和PINCH可能在结直肠癌发生发展过程中共同发挥作用。
Objective： To study the nucleoporin 88 （Nup88） and PINCH mRNA levels in eoloreetal carcinoma and cell lines, and the correlation between them thereof. Methods： The expression levels of Nap88 and PINCH mRNA were detected by RT-PCR in 47 samples of colorectal carcinoma, 44 adjacent non-cancerous mucosa, 45 proximal surgical margin, 44 distal surgical margin and 6 colon cell lines. The correlation study of Nup88 and PINCH was carried out by Spearman analysis. Results： The expression levels of Nup88 and PINCH mRNA were significantly higher in colorectal carcinoma group than those of adjacent non-cancerous mueosa, proximal and distal surgical margin. Besides, the expression levels of Nup88 and PINCH mRNA were positive correlated in four groups （rs=0.31, rs=0.35, rs=0.46 and rs=0.48）. Conclusion： Nup88 and PINCH might play a role in the tumorigenesis and development of colorectal cancer.
出处
《天津医药》
CAS
北大核心
2012年第9期870-872,共3页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(项目编号:30772115)
