摘要
目的:采用近红外光谱仪透射光谱技术对赤芍提取过程中芍药苷的含量进行检测分析。方法:将54份样品的原始光谱经多元散射校正(MSC)预处理,选取6 101.9～5 446.2,4 601.5～4 424.1 cm-1波段,并结合偏最小二乘法(PLS)对芍药苷建立定量校正模型并分析。结果:R2=0.998 9,内部交叉验证均方差(RMSECV)为0.025 2,最佳维数为6。用建立的校正模型对13份提取液样品中的芍药苷进行了预测,预测误差均方差(RMSEP)为0.086 1。结论:该方法分析快速、简便,结果准确可靠,对中药提取工艺优化和生产工艺过程的质量控制具有很好的应用前景。
Objective： To develop a novel method for fast analysis of one active component in water extracting process of Paeonia lactiflora with near infrared transmittance spectroscopy. Method： The calibration model of 54 extraction samples was developed by the spectral data pretreatment of the muhiplicative scatter correction （MSC） with the spectral regions （6 101.9-5 446.2, 4 601.5-4 424. 1 cm-1） and analyzed the correlation between the spectra and the corresponding values by PLS method. Result： The R2was 0. 998 9, the root mean square error of Cross-Validation （RMSECV） was 0. 025 2 and the best dimension was six. The paeoniflorin in the 13 samples was evaluated by using the model established, root mean square errors of prediction （RMSEP） was 0. 086 1. Conclusion： This method is not only rapid, simple, accurate and reliable, but also has great application prospects in the optimization of extraction technology and on-line monitoring of production process in TCM field.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期134-137,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
近红外透射光谱
赤芍
芍药苷
提取过程监测
多元散射校正
process monitoring
the near infrared transmittance spectroscopy
Paeonia lactiflora
paeoniflorin
extracting muhiplicative scatter correction （MSC）
