摘要
目的 观察汉防己甲素 (Tetrandrine,Tet)对实验性急性胰腺炎 (AP)的治疗效果 ,并对其作用机制进行探讨。方法 取 176只SD大鼠 ,雌雄不拘 ,随机分为 6组 ,经胰胆管逆行注射 3%牛磺脱氧胆酸钠 0 1ml/ 10 0 g体重 ,制成急性胰腺炎模型 ,记录 4h、8h腹水量、胰腺出血坏死面积的变化 ;测定血浆中钙离子浓度和淀粉酶活性、胰腺组织匀浆的丙二醛 (MDA)的含量 ;肺匀浆及腹水中PLA2活性的变化。光镜下观察胰组织的病理变化 ;记录动物的存活时间及 2 4h死亡率。结果 Tet能有效减轻AP大鼠胰腺的病理性损伤 ;减少腹水的生成量 ;与NS组相比 ,Tet组血浆中钙离子浓度进行性下降的程度减轻 ,淀粉酶活性降低、胰腺组织匀浆的MDA含量下降 ;Tet可降低AP大鼠的死亡率 ,延长动物的生存时间。结论 汉防己甲素可以通过抗氧自由基的生成、减轻组织过氧化损伤以及钙通道拮抗作用、减轻胰腺腺泡细胞钙超载而对急性胰腺炎发挥保护作用。
Objective To Investigate the effect of tetrandrine on sodium deoxytaurocholate induced acute pancreatitis (AP) in rats and explore its possible mechanism. Methods 176 Sprague Dawley rats both male and female were divided into six groups. AP models was induced by injecting 3% sodium deoxytaurocholate (1ml/kg weight) into the biliopancreatic duct except the rats of sham operation group. 10 minitues after operation, rats were injected intraperitoneally with saline, Tetrandrine or verapamil, respectively. The volume of ascites, bleeding and necrotic area of pancreas were measured at 4h and 8h. The amylase activity and calcium concentration in plasma and content of MDA in pancreas were determined. The survival time and motality of 11 rats different groups was recorded, and the pathology of pancreas was observed under microscope. Results Tetrandrine reduced the mortality (P<0.05) in 24h, and prolonged significantly the survival time of rats with AP(P<0.01), reduced ascites volume, ameliorated the inflammatory injury of MDA, and amylase activity of pancreas and attenuated hypocalcemia. The effects were similar to that of Verapamil (P>0.05). Conclusions The results of present study suggested that Tetrandrine possesses a therapeutic effect on the AP induced by sodium deoxytaurocholate in rats, in which the mechanism might be due to prevent from the production of reactive oxygen metablites, attenuate the injury of peroxidation, and block the calcium channal that decrease the overload of Ca 2+ in pancreatic acinus.
出处
《合肥医学院学报》
2000年第2期91-95,共5页
Journal of Zunyi Medical University
基金
贵州省科委科研基金资助项目
