摘要
目的检测HBV感染产妇乳汁中HBV-M和HBV-DNA,以评价母乳喂养的安全性。方法采用ELISA静置式与振动式孵育法定性检测乳汁HBsAg,同时采用荧光定量PCR法检测血清和乳汁HBV-DNA。结果 144例产妇血清HBV-DNA阳性率52.1%(75/144),乳汁阳性率34.7%(50/144),乳汁HBsAg静置式孵育法阳性66例(45.8%),振动式孵育为75例(52.1%)。结论应尽可能同时检测乳汁的HBsAg和HBV-DNA以提高母乳喂养的安全性。无HBV-DNA检测条件的实验室在检测乳汁中HBsAg时应采用振动式孵育以提高阳性检出率。
Objective To detect HBV - M and HBV - DND in the lac feminium of HBV - infected pureperas in order to eval- uate the safety of breast feeding. Methods ELISA standing type and oscillator type brooding - methods were adopted to detect HBsAg in the lac feminium qualifivly while fluorescent quantitation PCR method was applied to detect HBV - DNA in the serum and lac femini- urn. Resluts The positive rate of HBV - DNA was 52.1% （75/144） in the serum of 144 HBV - infected puerperas and 34.7% （50/ 144） in the lac feminium. Moreover,66（45.8% ）cases of positive HBsAg were detected by the standing type of brooding - method and 75 （ 52.1% ） by the oscillator type brooding - method. Conclusions HBsAg and HBV - DNA in the lac feminium should be detected simultaneously to improve the safety of breast feeding. Oscillator type brooding - method should be adopted to detect lac feminium to enhance the positive rate of HBsAg in laboratories unable to detect HBV -DNA.
出处
《武警医学》
CAS
2012年第8期692-693,696,共3页
Medical Journal of the Chinese People's Armed Police Force
