摘要
目的 :探讨建立人鼻粘膜呼吸区上皮细胞原代培养模型和纤毛运动频率测量的方法。方法 :利用酶消化法分离、以无血清生长因子培养基培养人鼻粘膜上皮细胞。用电视显微镜法观察并记录纤毛运动。结果 :培养的鼻粘膜呼吸上皮细胞在接种后 2 4 h贴壁 ,6～ 8d汇合 ,成活 1 6d,因实验需要结束培养。纤毛细胞纤毛摆动活跃 ,杯状细胞既分泌酸性粘多糖也分泌中性粘多糖 ,所培养细胞 2 3对 46条染色体正常。用电视显微镜法测得 2 9例1 45个人鼻粘膜细胞的纤毛运动频率均值为 ( 4 1 1± 2 4 )次 /min。结论 :酶消化法分离、无血清生长因子培养基培养 ,可以建立人鼻粘膜呼吸区上皮细胞的原代培养模型 ;
Objective:To establish a culture model of human nasal respiratory epithelial cells and a method of measuring human nasal ciliary motility.Method:The human nasal respiratory epithelial cells were detached with collagenase and cultured in serum free medium supplemented with hormones and growth factors,the ciliary beat frequency was measured by videomicroscopy.Result:After inoculation,cells cultured with this method adhered in 24 hours,confluented in 6～8 days and lived for 16 days.During that time ciliary beating was active,both acidic and neutral mucoitin granules were rich in goblet cells and all chromosome of 23 pairs were normal,the ciliary beat frequency in 29 subjects′nasal mucosa was(411±24)beats/min(±s).Conclusion:A culture model of human nasal respiratory epithelial cells in serum free medium supplemented with hormones and growth factors and a method of measuring human nasal ciliary motility was successfully established.
出处
《临床耳鼻咽喉科杂志》
CSCD
2000年第8期370-372,共3页
Journal of Clinical Otorhinolaryngology
关键词
鼻粘膜
上皮细胞
纤毛运动
细胞培养
Nasal mucosa Epithelial cells Ciliary motility Cultured cells
