摘要
目的 :探讨IL 6和B7双基因转染的小鼠肿瘤疫苗是否具有协同的抗肿瘤效应。方法 :利用含小鼠B7分子和人IL 6分子cDNA的逆转录病毒载体pLmB7SN和 pLhIL 6SN分别转染包装细胞株CRIP ,经G418抗性筛选后以含有B7或IL 6外源基因的病毒颗粒感染小鼠EL 4胸腺瘤细胞 ,观察EL 4 IL 6、EL 4 B7、EL 4 IL 6 +B7细胞在同基因宿主体内的致瘤性及作为抗肿瘤疫苗的能力。结果 :相对亲本肿瘤细胞EL 4而言 ,单基因和双基因转染的EL 4细胞在小鼠体内的生长速度均明显减慢 ,小鼠存活时间均有一定延长 ,3种转基因的EL 4细胞经X 射线灭活后免疫小鼠 ,虽均未能阻止随后接种的EL 4细胞的生长 ,但小鼠存活时间都明显延长 ,IL 6和B7双基因转染的EL 4肿瘤细胞在抗肿瘤活性方面与IL 6或B7单基因转染的EL 4细胞差异无显著性。结论 :与单基因表达疫苗比较 。
Objective: To explore whether co expression of IL 6 and B7 in EL 4 tumor cells could initiate an synergistic antitumor effect. Methods: The retroviral vectors pLmB7SN and pLhIL 6SN were transfected into the packaging cell line CRIP and the virus particles containing IL 6 or B7 gene were used to infect EL 4 tumor cells, obtaining the cell lines EL 4 IL 6, EL 4 B7 and EL 4 IL 6+B7. B6 mice were injected subcutaneously with various EL 4 transfectant cells and tumor growth conditions were observed. The vaccine potency of the above engineered EL 4 variants was analyzed. Results: When mice were immunized with the above irradiated EL 4 transfectant cells and challenged 7 days later with parental EL 4 cells, the survival time of mice was significantly prolonged. However, there is no difference between the survival time of mice vaccination with EL 4 cells transfected with double IL 6+B7 genes and single IL 6 or B7 gene. Conclusion: No synergistic antitumor immunity was observed in double IL 6+B7 genes transfected EL 4 cells.
出处
《北京医科大学学报》
CSCD
2000年第4期354-357,共4页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金!(394706 56)资助课题
