摘要
目的 实验研究克栓酶的抑瘤作用 ,以期扩展其药用范围 ,为治疗肿瘤增加药源。方法 采用双层琼脂培养 ,流式细胞检测 ,免疫组化图象分析 ,MTT ELISA等 ,以及荷瘤裸鼠整体实验方法观察对人肺癌细胞 (PG)与正常人胚内皮细胞增殖抑制作用。结果 证明克栓梅可抑制PG与人胚内皮细胞增殖 ,具有统计学意义。结论 克栓酶毒性低具有治疗肿瘤的潜能。在肿瘤综合治疗中如何合理联合配伍使用克栓酶值得进一步探讨。
Objective To research the Batroxobin DF 521 effect on inhibition of tumor in order to extend its useful range and provide a new drug for curing tumor.Methods To observe the Batroxobin DF 521 effect on human lung cancer cell line PG and normal human embryo vasculo endothelia cell using double layer agar culture,fluorecetometry,immunohisto chemical immage analysis,MTT ELISA and inhibitory tumor test with nude mice.Results The Batroxobin was able to inhibit amplification of PG and vasculo endothelia cells.Conclusion The Batroxobin is a low toxicial drug and has potential in curing tumor.It is worth to explore further how to appropriately use the Batroxobin with other drugs in tumor synthetical treatment.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第4期256-259,共4页
Cancer Research on Prevention and Treatment
关键词
克栓酶
抑瘤作用
PG细胞
实验研究
Batroxobin
Inhibitory tumor
PG cell
Embryo vasculo endothelia cell
