摘要
目的:研究姜黄素对A549细胞凋亡的诱导作用,并探讨其可能的分子机制。方法:姜黄素处理A549细胞后,MTT法于不同时间点检测A549细胞的增殖情况;姜黄素处理A549细胞48h后,流式细胞术(Flow CytoMetry,FCM)检测A549细胞的凋亡率;30μmol/L姜黄素处理A549细胞24h、48h、72h后,RT-PCR、Western-Blot检测A549细胞中P53、Bcl2、Bax和Caspase-3基因的表达水平。结果:姜黄素对肺癌A549细胞增殖的抑制作用具有显著的浓度-时间依赖关系,30μmol/L姜黄素可诱导A549细胞凋亡。30μmol/L姜黄素作用A549细胞24h即可引起A549细胞中P53、Bax和Caspase-3表达水平的上调,48h后可引起Bcl2表达水平的下调,诱导A549细胞凋亡。结论:30μmol/L姜黄素可诱导A549细胞凋亡,其机制可能与促进P53、Bax和Caspase-3基因的表达,抑制Bcl2基因的表达有关。
Objective：To study the induced effect of curcumin on apoptosis in Lung Cancer 3-549 Cells and explore the possible mechanisms. Methods： The proliferation of A549 cells was detected by MTT assay at different time points after curcumin treatment; the apoptosis of A549 cells was detected by FCM at 48 hours after curcumin treatment. The expression levels of P53, Bcl2, Bax, Caspase - 3 were detected by RT - PCR, Western - Blot at 48 hours after curcumin treatment. Result： Inhibitory effect of curcumin on proliferation in Lung Cancer 3.549 Cells has a significant concentration- time dependent manner. 30μmol/L can induce the apoptosis of A549 cells. The expression levels of P53, Bax and Caspase - 3 on A549 cells were up - regulation at 24 hours after 30μmol / L curcumin treatment; the expression level of Bcl2 on A549 cells was down - regulation at 48 hours after 30μmol/L curcumin treatment; the apoptosis of A549 cells was induced at 48 hours after 30μmol/L curcumin treatment. Conclusion：30μmol/L curcumin can induce the apoptosis of A549 cells, the mechanism may be related to the promotion of P53, Bax and Caspase - 3 gene expression, inhibition of Bcl2 gene expression.
出处
《激光杂志》
CAS
CSCD
北大核心
2012年第4期58-60,共3页
Laser Journal
