摘要
目的探讨基因检测技术和显微镜观察药物敏感度检测技术(MODS)对结核分枝杆菌(MTB)耐左氧氟沙星(LFX)快速诊断价值。方法选取MTB标准株(H37Rv)和32株耐LFX临床分离株,行gyrA基因喹诺酮类药物耐药决定区(QRDR)序列测定,同时,用24孔细胞培养板进行MTB液体药敏检测。结果基因检测结果显示,H37Rv未发生gyrA基因突变;与H37Rv株序列的差异比较显示,29株耐LFX临床株发生了有义突变,突变率90.6%。MODS与罗氏绝对浓度法药敏(L-J)比较,H37Rv完全相符;32株耐LFX临床分离株,相符31株,不符1株,符合率为96.9%。结论 gyrA基因突变检测可作为快速诊断结核分枝杆菌对LFX敏感性的一种方法,但不能完全区别低耐药和高耐药而使用受到限制。MODS技术检测LFX耐药性具有快速、操作简便、灵敏度和特异性高等优点,可作为结核分枝杆菌对LFX敏感性的快速检测新方法。
Objective To study the value of gene detection and micro-scopic observation drug susceptibility for rapid diagnosis of levofloxacin（LFX） resistance in Mycobacterium tuberculosis（MTB）.Methods H37Rv and 32 clinical isolates of LFX-resistant tuberculosis were studied.The gyrA genes were identified and the corresponding quinolone resistance determining region（QRDR） was sequenced.24-hole cell culture plate was used to detect the susceptibility of MTB to drugs in liquid medium.Results Gene mutation wasn’t found in H37Rv.29 isolates showed gyrA mutations and the mutation rate was 90.6%.Among the 32 MTB isolates resistant,31 isolates showed resistance to LFX and the concordance rates of susceptibility between MODS and L-J was 96.9%.Conclusion The GyrA mutation detection could be a rapid diagnosis method of LFX resistance in MTB,but could not totally identify low resistance and high resistance.MODS assay could be used for rapid screening of LFX resistance in MTB.
出处
《国际检验医学杂志》
CAS
2012年第13期1554-1555,1558,共3页
International Journal of Laboratory Medicine
基金
2010年江苏省南通市社会发展科技计划项目(S2010038)
关键词
分枝杆菌
结核
左氧氟沙星
敏感性试验
基因突变
显微镜观察药物敏感性技术
Mycobacterium tuberculosis； levofloxacin； sensitivity tests； gene mutation； microscopic observation drug susceptibility assay
